Lipid quantitation and metabolomics data from vitamin E-deficient and -sufficient zebrafish embryos from 0 to 120 hours-post-fertilization
| Autor: | Robert L. Tanguay, Melissa McDougall, Maret G. Traber, Hye Kyeong Kim, Enrique Cadenas, J. Frederik Stevens, Jaewoo Choi, Gerd Bobe |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty animal structures medicine.medical_treatment Danio Biology lcsh:Computer applications to medicine. Medical informatics Choline 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Human fertilization Internal medicine medicine Ferroptosis lcsh:Science (General) Zebrafish Data Article Multidisciplinary Vitamin E fungi Embryo biology.organism_classification Docosahexaenoic acid 030104 developmental biology Endocrinology Glucose chemistry embryonic structures Alpha-tocopherol lcsh:R858-859.7 alpha-Tocopherol 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Data in Brief, Vol 11, Iss C, Pp 432-441 (2017) Data in Brief |
| ISSN: | 2352-3409 |
| Popis: | The data herein is in support of our research article by McDougall et al. (2017) [1], in which we used our zebrafish model of embryonic vitamin E (VitE) deficiency to study the consequences of VitE deficiency during development. Adult 5D wild-type zebrafish (Danio rerio), fed defined diets without (E-) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet), were spawned to obtain E- and E+ embryos that we evaluated using metabolomics and specific lipid analyses (each measure at 24, 48, 72, 120 hours-post-fertilization, hpf), neurobehavioral development (locomotor responses at 96 hpf), and rescue strategies. Rescues were attempted using micro-injection into the yolksac using VitE (as a phospholipid emulsion containing d6-α-tocopherol at 0 hpf) or D-glucose (in saline at 24 hpf). |
| Databáze: | OpenAIRE |
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