Influence of gender and interleukin-10 deficiency on the inflammatory response during lung infection withPseudomonas aeruginosain mice
| Autor: | Danuta Radzioch, Claude Lachance, Peter K. Stotland, Claudine Guilbault, Thomas A. Hamilton, David H. Eidelman, Mifong Tam, Anna Keller, Mary M. Stevenson, Elizabeth A. Cowley, Luann Thompson-Snipes |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_treatment Immunology Biology medicine.disease_cause Cystic fibrosis Mice Sex Factors medicine Animals Immunology and Allergy Pseudomonas Infections Lung Respiratory Tract Infections Mice Knockout medicine.diagnostic_test Tumor Necrosis Factor-alpha Pseudomonas aeruginosa Body Weight Interleukin Original Articles medicine.disease Interleukin-10 Mice Inbred C57BL Survival Rate Interleukin 10 Bronchoalveolar lavage Cytokine medicine.anatomical_structure Knockout mouse Disease Progression Female Disease Susceptibility Bronchoalveolar Lavage Fluid |
| Zdroj: | Immunology. 107:297-305 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.2002.01508.x |
| Popis: | Cystic fibrosis females have a worse prognosis compared to male patients. Furthermore, cystic fibrosis patients infected with Pseudomonas aeruginosa have been shown to have dysregulated cytokine profiles, as higher levels of tumour necrosis factor alpha (TNF-alpha), interleukin (IL)-8, and lower levels of IL-10 are found in the bronchoalveolar lavage fluid compared to healthy controls. The present study was aimed at investigating the importance of gender and IL-10 in the susceptibility of C57BL/6 mice to pulmonary infection with Pseudomonas aeruginosa. We found that wildtype females were more susceptible than males to infection, as we observed greater weight loss, higher bacterial load, and inflammatory mediators in their lungs. IL-10 knockout mice, both females and males, had higher levels of TNF-alpha in the lungs compared to wildtype mice and maintained higher levels of polymorphonuclear cells and lower levels of macrophages for a longer period of time. Our results demonstrate that the number of bacteria recovered from the lungs of IL-10 knockout male mice was significantly higher than that observed in their wildtype male counterparts and we show that neutralization of IL-10 in infected female mice for a prolonged period of time leads to increased susceptibility to infection. Results reported in this study clearly demonstrate that females, both wildtype and IL-10 knockout mice are more susceptible to Pseudomonas aeruginosa infection than males, and that they mount a stronger inflammatory response in the lungs. |
| Databáze: | OpenAIRE |
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