Computational model-informed design and bioprinting of cell-patterned constructs for bone tissue engineering
| Autor: | Aurélie Carlier, Bahattin Koc, Forough Hafezi, Gözde Akdeniz Skvortsov, Jennifer Patterson, Hans Van Oosterwyck, Eleonora Ferraris |
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| Přispěvatelé: | RS: MERLN - Cell Biology - Inspired Tissue Engineering (CBITE), CBITE |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Engineering TA164 Bioengineering Bone Regeneration cell pattern Cell Survival Biomedical Engineering Bioengineering Matrix (biology) Biochemistry TA401-492 Materials of engineering and construction. Mechanics of materials Bone tissue engineering Bone and Bones Biomaterials 03 medical and health sciences Mice Tissue engineering Animals Bone regeneration bone tissue engineering Process (anatomy) Cell Proliferation cell-laden hydrogels TP0248.13 Biotechnology Tissue Engineering Tissue Scaffolds business.industry Regeneration (biology) Computational Biology Hydrogels General Medicine non-healing bone defects Fibroblasts computational model 030104 developmental biology R858-859.7 Computer applications to medicine. Medical informatics Self-healing hydrogels Printing Three-Dimensional NIH 3T3 Cells business R855-855.5 Medical technology bioprinting Biotechnology Biofabrication Biomedical engineering |
| Zdroj: | Biofabrication, 8(2):025009. IOP Publishing Ltd. |
| ISSN: | 1758-5082 |
| Popis: | Three dimensional (3D) bioprinting is a rapidly advancing tissue engineering technology that holds great promise for the regeneration of several tissues, including bone. However, to generate a successful 3D bone tissue engineering construct, additional complexities should be taken into account such as nutrient and oxygen delivery, which is often insufficient after implantation in large bone defects. We propose that a well-designed tissue engineering construct, that is, an implant with a specific spatial pattern of cells in a matrix, will improve the healing outcome. By using a computational model of bone regeneration we show that particular cell patterns in tissue engineering constructs are able to enhance bone regeneration compared to uniform ones. We successfully bioprinted one of the most promising cell-gradient patterns by using cell-laden hydrogels with varying cell densities and observed a high cell viability for three days following the bioprinting process. In summary, we present a novel strategy for the biofabrication of bone tissue engineering constructs by designing cell-gradient patterns based on a computational model of bone regeneration, and successfully bioprinting the chosen design. This integrated approach may increase the success rate of implanted tissue engineering constructs for critical size bone defects and also can find a wider application in the biofabrication of other types of tissue engineering constructs. |
| Databáze: | OpenAIRE |
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