Functional analysis of Pdp1 and vrille in the circadian system of a cricket
| Autor: | Motoki Nose, Yumina Narasaki-Funo, Kenji Tomioka, Yasuaki Tomiyama, Tetsuya Bando |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine Physiology Period (gene) Circadian clock 01 natural sciences Gryllidae 03 medical and health sciences Rhythm Cricket RNA interference Circadian Clocks Animals Circadian rhythm biology Gryllus bimaculatus Optic Lobe Nonmammalian biology.organism_classification Cell biology CLOCK 010602 entomology Basic-Leucine Zipper Transcription Factors 030104 developmental biology Insect Science Insect Proteins RNA Interference |
| Zdroj: | Journal of Insect Physiology. 127:104156 |
| ISSN: | 0022-1910 |
| DOI: | 10.1016/j.jinsphys.2020.104156 |
| Popis: | Circadian rhythms are generated by a circadian clock for which oscillations are based on the rhythmic expression of the so-called clock genes. The present study investigated the role of Gryllus bimaculatus vrille (Gb’vri) and Par domain protein 1 (Gb’Pdp1) in the circadian clock of the cricket Gryllus bimaculatus. Structural analysis of Gb’vri and Gb’Pdp1 cDNAs revealed that they are a member of the bZIP transcription factors. Under light/dark cycles (LD) both genes were rhythmically expressed in the clock tissue, the optic lobes, whereas the rhythm diminished under constant darkness (DD). Gb’vri and Gb’Pdp1 mRNA levels were significantly reduced by RNA interference (RNAi) of Gb’Clk and Gb’cyc, suggesting they are controlled by Gb’CLK/Gb’CYC. RNAi of Gb’vri and Gb’Pdp1 had little effect on locomotor rhythms, although their effects became visible when treated together with Gb’cycRNAi. The average free-running period of Gb’vriRNAi/Gb’cycRNAi crickets was significantly shorter than that of Gb’cycRNAi crickets. A similar period shortening was observed also when treated with Gb’Pdp1RNAi/Gb’cycRNAi. Some Gb’Pdp1RNAi/Gb’cycRNAi crickets showed rhythm splitting into two free-running components with different periods. Gb’vriRNAi and Gb’Pdp1RNAi treatments significantly altered the expression of Gb’Clk, Gb’cyc, and Gb’tim in LD. These results suggest that Gb’vri and Gb’Pdp1 play important roles in cricket circadian clocks. |
| Databáze: | OpenAIRE |
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