Kinetics of viral load and antibody response in relation to COVID-19 severity
Autor: | Lijun Kuang, Liyan Wen, Ling Sang, Rongchang Chen, Nanshan Zhong, Feng Ye, Yanjun Zhang, Jingxian Zhao, Xiaofang Huang, Zhao Chen, Airu Zhu, Jieling Liang, Abeer N. Alshukairi, Mian Gan, Haitao Tan, Yanqun Wang, Zhengtu Li, Manal M. Al Gethamy, Tie Song, Yongbo Huang, Jianfen Zhuo, Zhen Zhuang, Ling Luo, Shicong Ruan, Jincun Zhao, Min Li, Yimin Li, Jicheng Huang, J Wang, Lan Tang, Zhaoyong Zhang, Yimin Lin, Jing Sun, J. S. Malik Peiris, Chris Ka Pun Mok, Chunke Chen, Yongliang Jiang, Fang Li, Lu Zhang, Yuming Li, Bei Zhong, Stanley Perlman, Huibin Lv, Ying Deng |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
viruses Pneumonia Viral Antibodies Viral Severe Acute Respiratory Syndrome medicine.disease_cause Virus Betacoronavirus 03 medical and health sciences 0302 clinical medicine Intensive care medicine Humans Viral shedding Pandemics COVID-19 Serotherapy Coronavirus biology SARS-CoV-2 business.industry Immunization Passive COVID-19 General Medicine Viral Load medicine.disease Kinetics 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Antibody Formation Immunology biology.protein Middle East respiratory syndrome Antibody Coronavirus Infections business Viral load Research Article Respiratory tract |
Zdroj: | J Clin Invest |
ISSN: | 1558-8238 0021-9738 |
Popis: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent for coronavirus 2019 (COVID-19) pneumonia. Little is known about the kinetics, tissue distribution, cross-reactivity, and neutralization antibody response in patients with COVID-19. Two groups of patients with RT-PCR–confirmed COVID-19 were enrolled in this study: 12 severely ill patients in intensive care units who needed mechanical ventilation and 11 mildly ill patients in isolation wards. Serial clinical samples were collected for laboratory detection. Results showed that most of the severely ill patients had viral shedding in a variety of tissues for 20–40 days after onset of disease (8/12, 66.7%), while the majority of mildly ill patients had viral shedding restricted to the respiratory tract and had no detectable virus RNA 10 days after onset (9/11, 81.8%). Mildly ill patients showed significantly lower IgM response compared with that of the severe group. IgG responses were detected in most patients in both the severe and mild groups at 9 days after onset, and remained at a high level throughout the study. Antibodies cross-reactive to SARS-CoV and SARS-CoV-2 were detected in patients with COVID-19 but not in patients with MERS. High levels of neutralizing antibodies were induced after about 10 days after onset in both severely and mildly ill patients which were higher in the severe group. SARS-CoV-2 pseudotype neutralization test and focus reduction neutralization test with authentic virus showed consistent results. Sera from patients with COVID-19 inhibited SARS-CoV-2 entry. Sera from convalescent patients with SARS or Middle East respiratory syndrome (MERS) did not. Anti–SARS-CoV-2 S and N IgG levels exhibited a moderate correlation with neutralization titers in patients’ plasma. This study improves our understanding of immune response in humans after SARS-CoV-2 infection. |
Databáze: | OpenAIRE |
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