Correlation between radical scavenging capacity and carotenoid profile during Pleoticus muelleri larval development
| Autor: | Susana María Velurtas, Ana Cristina Díaz, Jorge L. Fenucci, Sara Noemí Mendiara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
DPPH
Ontogeny Radical Biology Ciencias Biológicas chemistry.chemical_compound SCAVENGING CAPACITY Astaxanthin LARVAL REARING Food science Carotenoid chemistry.chemical_classification Bioquímica y Biología Molecular biology.organism_classification Crustacean Hatchery chemistry Mysis Biochemistry CRUSTACEA CAROTENOID Animal Science and Zoology PENAEOIDEA CIENCIAS NATURALES Y EXACTAS Developmental Biology |
| Popis: | The purpose of this study was to measure the concentration of carotenoids and properties which occur in tissues to neutralize free radicals during ontogeny of Pleoticus muelleri. The stages of nauplius, protozoea, mysis and postlarvae of 1, 6, 10, 26, 30 days were examined from hatchery raised postlarvae from wild females. The β-carotene and the astaxanthin from the lyophilized tissue were quantified using a ultraviolet-visible spectrophotometer. Free radicals scavenging properties of tissues extracts were evaluated by electron paramagnetic resonance (EPR). The reaction of protective substances was followed with the 1,1-diphenyl-2- picryhydrazyl (DPPH) radical. The concentration of carotenoids of the whole larvae and postlarvae ranged from 1.72 to 87.04 μg g-1 for β-carotene, and 2.26 to 19.86 μg g-1 for astaxanthin. All the larval and postlarvae stages showed a protective capacity. In the stages of mysis, postlarvae 1 and 30 the homogenates were monitored without DPPH. The undefined EPR signal was quantified and considered as a "pool" of persistent radicals, with a concentration about 10-5 M. A relationship was observed between the concentration of carotenoids and the protective capacity of the homogenate. The postlarvae stages had a high concentration of carotenoids and the greatest protective capacity. Fil: Díaz, Ana Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina Fil: Velurtas, Susana María. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina Fil: Mendiara, Sara Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Ciencias Marinas; Argentina Fil: Fenucci, Jorge Lino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|