Evidences of Changes in Surface Electrostatic Charge Distribution during Stabilization of HPV16 Virus-Like Particles
| Autor: | Ernesto Vicente-Alique, Y. Wang, Javier Martínez-Salazar, Rafael Núñez-Ramírez, Juan Francisco Vega |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España), Sino Biological, SCOAP |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Light lcsh:Medicine Pathology and Laboratory Medicine Light scattering Viral Packaging Scattering Mathematical and Statistical Techniques Electricity Medicine and Health Sciences Static light scattering Electron Microscopy lcsh:Science Mass Diffusivity Microscopy Human papillomavirus 16 Multidisciplinary Physics Electromagnetic Radiation Classical Mechanics Electrostatics Chemistry Chemical physics Medical Microbiology Viral Pathogens Autocorrelation Physical Sciences Viruses Engineering and Technology Pathogens Statistics (Mathematics) Research Article Electrophoresis Materials science Papillomaviruses Static Electricity Fluid Mechanics Research and Analysis Methods Electric charge Microbiology Continuum Mechanics HPV-16 03 medical and health sciences Dynamic light scattering Virology Surface charge Statistical Methods Microbial Pathogens Chemical Physics Biology and life sciences lcsh:R Organisms Light Scattering Virion Fluid Dynamics Viral Replication Dynamic Light Scattering 030104 developmental biology Signal Processing Hydrodynamics lcsh:Q Transmission Electron Microscopy Electrophoretic light scattering DNA viruses Mathematics |
| Zdroj: | PLoS ONE Digital.CSIC. Repositorio Institucional del CSIC instname PLoS ONE, Vol 11, Iss 2, p e0149009 (2016) |
| ISSN: | 1932-6203 |
| Popis: | 17 págs.; 6 figs. ; Open Access funded by Creative Commons Atribution Licence 4.0 The stabilization of human papillomavirus type 16 virus-like particles has been examined by means of different techniques including dynamic and static light scattering, transmission electron microscopy and electrophoretic mobility. All these techniques provide different and often complementary perspectives about the aggregation process and generation of stabilized virus-like particles after a period of time of 48 hours at a temperature of 298 K. Interestingly, static light scattering results point towards a clear colloidal instability in the initial systems, as suggested by a negative value of the second virial coefficient. This is likely related to small repulsive electrostatic interactions among the particles, and in agreement with relatively small absolute values of the electrophoretic mobility and, hence, of the net surface charges. At this initial stage the small repulsive interactions are not able to compensate binding interactions, which tend to aggregate the particles. As time proceeds, an increase of the size of the particles is accompanied by strong increases, in absolute values, of the electrophoretic mobility and net surface charge, suggesting enhanced repulsive electrostatic interactions and, consequently, a stabilized colloidal system. These results show that electrophoretic mobility is a useful methodology that can be applied to screen the stabilization factors for virus-like particles during vaccine development. This research work was supported by Spanish Ministry of Economy and Competitiveness (MINECO), Project MAT2012-36341, and by the CSIC, Project PIE201360E097. Sino Biological, Inc., provided support in the form of salaries for author YW, but did not have any additional role, such as in the study design or data collection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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