Noncoding RNAs prevent spreading of a repressive histone mark
| Autor: | Raghavendran Kulasegaran-Shylini, Yukiko Shimada, Claudia Keller, Hans-Rudolf Hotz, Marc Bühler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Chromatin Immunoprecipitation
RNA Untranslated Euchromatin Chromosomal Proteins Non-Histone Heterochromatin Blotting Western Molecular Sequence Data Cell Cycle Proteins Real-Time Polymerase Chain Reaction Methylation Histones 03 medical and health sciences 0302 clinical medicine Structural Biology Schizosaccharomyces Endoribonucleases RNA-Induced Silencing Complex Histone code Small nucleolar RNA Molecular Biology 030304 developmental biology 0303 health sciences Base Sequence biology Histone-Lysine N-Methyltransferase Methyltransferases Sequence Analysis DNA Argonaute Blotting Northern biology.organism_classification Long non-coding RNA Protein Structure Tertiary Cell biology Gene Expression Regulation 030220 oncology & carcinogenesis Argonaute Proteins Schizosaccharomyces pombe RNA Long Noncoding Heterochromatin protein 1 Schizosaccharomyces pombe Proteins |
| Zdroj: | Nature Structural & Molecular Biology |
| ISSN: | 1545-9993 |
| DOI: | 10.1038/nsmb1113-1340 |
| Popis: | Transcription of eukaryotic genomes is more widespread than was previously anticipated and results in the production of many non-protein-coding RNAs (ncRNAs) whose functional relevance is poorly understood. Here we demonstrate that ncRNAs can counteract the encroachment of heterochromatin into neighboring euchromatin. We have identified a long ncRNA (termed BORDERLINE) that prevents spreading of the HP1 protein Swi6 and histone H3 Lys9 methylation beyond the pericentromeric repeat region of Schizosaccharomyces pombe chromosome 1. BORDERLINE RNAs act in a sequence-independent but locus-dependent manner and are processed by Dicer into short RNAs referred to as brdrRNAs. In contrast to canonical centromeric short interfering RNAs, brdrRNAs are rarely loaded onto Argonaute. Our analyses reveal an unexpected regulatory activity of ncRNAs in demarcating an epigenetically distinct chromosomal domain that could also be operational in other eukaryotes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|