Altered small intestinal absorptive enzyme activities in leptin-deficient obese mice: influence of bowel resection
| Autor: | Deborah A. Swartz-Basile, Carol L. Svatek, Henry A. Pitt, Jae H. Noh, James M. Kiely |
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| Rok vydání: | 2006 |
| Předmět: |
Leptin
Male medicine.medical_specialty medicine.medical_treatment Ileum Dipeptidyl peptidase Sucrase Mice Internal medicine Diabetes mellitus Intestine Small Medicine Animals Obesity Dipeptidyl peptidase-4 Leptin Deficiency business.industry digestive oral and skin physiology General Medicine Bowel resection medicine.disease Mice Inbred C57BL Endocrinology medicine.anatomical_structure Intestinal Absorption Pediatrics Perinatology and Child Health Surgery business hormones hormone substitutes and hormone antagonists Acyltransferases Peptide Hydrolases |
| Zdroj: | Journal of pediatric surgery. 41(7) |
| ISSN: | 1531-5037 |
| Popis: | Background Residual bowel increases absorption after massive small bowel resection. Leptin affects intestinal adaptation, carbohydrate, peptide, and lipid handling. Sucrase, peptidase, and acyl coenzyme A:monoacylglycerol acyltransferase (MGAT) are involved in carbohydrate, protein, and lipid absorption. We hypothesized that leptin-deficient obese mice would have altered absorptive enzymes compared with controls before and after small bowel resection. Methods Sucrase, peptidase (aminopeptidase N [ApN], dipeptidyl peptidase IV [DPPIV]), and MGAT activities were determined from lean control (C57BL/6J, n = 16) and leptin-deficient (Lepob, n = 16) mice small bowel before and after 50% resection. Results Ileal sucrase activity was greater in obese mice before and after resection. Jejunal ApN and DPPIV activities were lower for obese mice before resection; ileal ApN activity was unaltered after resection for both strains. Resection increased DPPIV activity in both strains. Jejunal MGAT in obese mice decreased postresection. In both strains, ileal MGAT activity decreased after resection, and obese mice had greater activity in remnant ileum. Conclusions After small bowel resection, leptin-deficient mice have increased sucrase activity and diminished ileal ApN, DPPIV, and MGAT activity compared with controls. Therefore, we conclude that leptin deficiency alters intestinal enzyme activity in unresected animals and after small bowel resection. Altered handling of carbohydrate, protein, and lipid may contribute to obesity and diabetes in leptin-deficient mice. |
| Databáze: | OpenAIRE |
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