R-sulforaphane modulates the expression profile of AhR, ERα, Nrf2, NQO1, and GSTP in human breast cell lines
| Autor: | Violetta Krajka-Kuźniak, Hanna Szaefer, Barbara Licznerska |
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| Rok vydání: | 2020 |
| Předmět: |
GSTP
NF-E2-Related Factor 2 Clinical Biochemistry Breast Neoplasms Article Nrf2 Breast cancer Isothiocyanates Cell Line Tumor Basic Helix-Loop-Helix Transcription Factors NAD(P)H Dehydrogenase (Quinone) medicine Anticarcinogenic Agents Humans skin and connective tissue diseases Adverse effect Molecular Biology Messenger RNA Chemistry AhR Estrogen Receptor alpha Cell Biology General Medicine medicine.disease Breast cells Glutathione S-Transferase pi Receptors Aryl Hydrocarbon Cell culture Sulfoxides Cancer research NQO1 Female Breast cancer cells Transcriptome (R)-sulforaphane Sulforaphane Human breast Homeostasis |
| Zdroj: | Molecular and Cellular Biochemistry |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/s11010-020-03913-5 |
| Popis: | Our previous study showed remarkable differences in the effect of R-sulforaphane (R-SFN) on the expression of CYPs 19, 1A1, 1A2, and 1B1 in ER(+) MCF7, ER( −) MDA-MB-231, and non-tumorigenic immortalized MCF10A (8). This study aimed to evaluate the effect of R-SFN on phase II enzymes induction and expression of AhR, Nrf2, and ERα in the same breast cell lines. The results showed increased expression of GSTP as a result of treatment with R-SFN in breast cancer cells. An increased NQO1 transcript and protein levels were found in all breast cells, with the most significant increase in MCF7 cells. Similarly, the enhancement of Nrf2 expression was noticed in all tested cells. AhR gene transcript and protein were decreased in MCF7 cells. In MDA-MB-231, increased AhR mRNA was not confirmed at the protein level. No differences were found in the expression of ERα. Overall, the results of the present study extended our earlier suggestions on the possible interference of R-SFN with estrogens homeostasis in breast cancer cells differing in ERα status, as well as in non-tumorigenic immortalized breast epithelial cells. While some of R-SFN effects might be beneficial and useful in breast cancer prevention, the others, particularly GSTP induction, may lead to adverse effects. |
| Databáze: | OpenAIRE |
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