Amphetamine Withdrawal Differentially Increases the Expression of Organic Cation Transporter 3 and Serotonin Transporter in Limbic Brain Regions
| Autor: | Kenneth J. Renner, Gina L. Forster, Jamie L. Scholl, Rajeshwari R. Solanki, Michael J. Watt |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology psychostimulant Hippocampus Serotonergic lcsh:RC321-571 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Amphetamine lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Serotonin transporter Original Research dorsomedial hypothalamus biology Kindling business.industry General Neuroscience Central nucleus of the amygdala anxiety serotonin 030104 developmental biology Endocrinology Hypothalamus central amygdala biology.protein Serotonin business ventral hippocampus 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Experimental Neuroscience Journal of Experimental Neuroscience, Vol 10 (2016) Journal of Experimental Neuroscience, Vol 2016, Iss 10, Pp 93-100 (2016) |
| ISSN: | 1179-0695 |
| DOI: | 10.4137/jen.s40231 |
| Popis: | Amphetamine withdrawal increases anxiety and stress sensitivity related to blunted ventral hippocampus (vHipp) and enhances the central nucleus of the amygdala (CeA) serotonin responses. Extracellular serotonin levels are regulated by the serotonin transporter (SERT) and organic cation transporter 3 (OCT3), and vHipp OCT3 expression is enhanced during 24 hours of amphetamine withdrawal, while SERT expression is unaltered. Here, we tested whether OCT3 and SERT expression in the CeA is also affected during acute withdrawal to explain opposing regional alterations in limbic serotonergic neurotransmission and if respective changes continued with two weeks of withdrawal. We also determined whether changes in transporter expression were confined to these regions. Male rats received amphetamine or saline for two weeks followed by 24 hours or two weeks of withdrawal, with transporter expression measured using Western immunoblot. OCT3 and SERT expression increased in the CeA at both withdrawal timepoints. In the vHipp, OCT3 expression increased only at 24 hours of withdrawal, with an equivalent pattern seen in the dorsomedial hypothalamus. No changes were evident in any other regions sampled. These regionally specific changes in limbic OCT3 and SERT expression may partially contribute to the serotonergic imbalance and negative affect during amphetamine withdrawal. |
| Databáze: | OpenAIRE |
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