Allogeneic Intrabone Marrow-Bone Marrow Transplantation plus Donor Lymphocyte Infusion Suppresses Growth of Colon Cancer Cells Implanted in Skin and Liver of Rats
| Autor: | Akio Shigematsu, Keiji Nakano, Naoko Koike-Kiriyama, Yasuhiko Kiyozuka, Yasushi Koike, Keizo Minamino, Yasushi Adachi, Yasuo Kamiyama, Susumu Ikehara, Masayoshi Iwasaki, Yasuhiro Suzuki, Hiromi Mukaide, Airo Tubura |
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| Rok vydání: | 2007 |
| Předmět: |
CD4-Positive T-Lymphocytes
Bone marrow transplantation Colorectal cancer Spleen CD8-Positive T-Lymphocytes Biology Donor lymphocyte infusion Rats Inbred BN medicine Animals Transplantation Homologous Fibrosarcoma Bone Marrow Transplantation Cell Proliferation Skin Cell Biology medicine.disease Xenograft Model Antitumor Assays Rats Inbred F344 Rats Killer Cells Natural Survival Rate surgical procedures operative medicine.anatomical_structure Liver Lymphocyte Transfusion Colonic Neoplasms Cancer cell Immunology Cancer research Molecular Medicine Bone marrow CD8 Developmental Biology |
| Zdroj: | Stem Cells. 25:385-391 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.2006-0227 |
| Popis: | We have recently found that allogeneic intrabone marrow-bone marrow transplantation (IBM-BMT) + donor lymphocyte infusion (DLI) using CD4+ cell-depleted spleen cells (CD4− cells) can prevent graft-versus-host disease (GvHD) but suppress tumor growth (Meth A: fibrosarcoma) in mice. In the present study, we show that allogeneic IBM-BMT + DLI using CD4− cells also has suppressive effects on the growth of colon cancer cells implanted not only in the skin but also in the liver of rats. First, we examined the effects of allogeneic IBM-BMT + DLI on the subcutaneously inoculated ACL-15 (rat colon cancer cell line). Lethally irradiated Fischer rats (F344 rats) were transplanted with T-cell-depleted bone marrow cells (BMCs) from Brown Norway (BN) rats. Simultaneously, DLI was performed using whole spleen cells (whole cells), CD4+ cell-depleted spleen cells (CD4− cells) or CD8+ cell-depleted spleen cells (CD8− cells) of BN rats. Although allogeneic IBM-BMT + DLI suppressed tumor growth, a considerable number of rats treated with allogeneic IBM-BMT + DLI using whole cells or CD8− cells died due to GvHD. In contrast, allogeneic IBM-BMT + DLI using CD4− cells also suppressed tumor growth, but there was no GvHD. Based on these findings, we next examined the effects of allogeneic IBM-BMT + DLI using CD4− cells on the cancer cells implanted in the liver. Allogeneic IBM-BMT + DLI using CD4− cells via the portal vein significantly prolonged the survival. These results suggest that allogeneic IBM-BMT + DLI using CD4− cells could become a new strategy for the treatment of solid tumors. |
| Databáze: | OpenAIRE |
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