MiR-330-5p regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma
| Autor: | Xiao-ning Zhang, Shu-wei Zhou, Cai-bin Gan, Bei-bei Su |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Skin Neoplasms Blotting Western Protein Disulfide-Isomerases Down-Regulation Biology Real-Time Polymerase Chain Reaction Cell Line law.invention 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement law microRNA Biomarkers Tumor medicine Humans Neoplasm Invasiveness Melanoma Cell Proliferation Monophenol Monooxygenase Cell growth medicine.disease Up-Regulation Gene Expression Regulation Neoplastic Blot MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Cell culture 030220 oncology & carcinogenesis Cancer research Suppressor Surgery |
| Zdroj: | Journal of Surgical Research. 203:434-440 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2016.03.021 |
| Popis: | Increasing evidence has suggested that miR-330-5p can function as a tumor suppressor in different types of cancers. However, the effects and underlying mechanisms of miR-330-5p in the development of cutaneous malignant melanoma (CMM) remain largely unknown. The aim of the present study was to investigate the role of miR-330-5p in CMM and to determine the molecular mechanisms underlying its action.The expression level of miR-330-5p was detected in 26 cases of primary CMM tissues and cell lines by real-time quantitative polymerase chain reaction. We also assessed whether overexpression of miR-330-5p influences in vitro cell proliferation, invasion, and migration. Western blotting analysis was used to detect the influence of miR-330-5p on the targets, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-330-5p in CMM tissues.Our study showed that miR-330-5p was downregulated in CMM tissues (P = 0.010) and cell lines (P 0.05), and patients with high mitotic activity showed lower miR-330-5p expression levels (P = 0.002). Enforced expression of miR-330-5p inhibits malignant CMM cells proliferation and migration and led to downregulation of the TYR and PDIA3 protein. Moreover, the expression level of miR-330-5p in CMM tissues showed inverse relationship with the expression level of TYR and PDIA3 protein.In conclusion, our findings suggested that miR-330-5p represents a potential tumor-suppressive miRNA and plays an important role in CMM progression by suppressing TYR and PDIA3 expression. |
| Databáze: | OpenAIRE |
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