Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
| Autor: | Kyoko Miura, Kumiko Iwabuchi, Takashi Shinohara, Masato Nakagawa, Tomoko Ichisaka, Masanori Imamura, Shinya Yamanaka, Mito Kanatsu-Shinohara, Jiyoung Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Genetic Markers
Male Chromatin Immunoprecipitation Octamer Transcription Factor-3 Organic Cation Transport Proteins Transcription Genetic Somatic cell Fibroblast Growth Factor 4 Biology Germline Mice SOX2 medicine Animals lcsh:QH301-705.5 Cells Cultured Regulation of gene expression Homeodomain Proteins Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Stem Cells Gene Expression Regulation Developmental DNA Nanog Homeobox Protein DNA Methylation Molecular biology Chromosomes Mammalian Spermatozoa DNA-Binding Proteins medicine.anatomical_structure lcsh:Biology (General) DNA methylation Trans-Activators Stem cell Germ cell Developmental Biology Research Article |
| Zdroj: | BMC Developmental Biology BMC Developmental Biology, Vol 6, Iss 1, p 34 (2006) |
| ISSN: | 1471-213X |
| Popis: | BackgroundWe previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells.ResultsIn all ECATs examined, the promoter region had low methylation levels in ES cells, but higher levels in somatic cells. In contrast, in spite of their lack of pluripotency, male germline stem (GS) cells expressed most ECATs and exhibited hypomethylation of ECAT promoter regions. We observed a similar hypomethylation of ECAT loci in adult testis and isolated sperm. Some ECATs were even less methylated in male germ cells than in ES cells. However, a few ECATs were not expressed in GS cells, and most of them targets of Oct3/4 and Sox2. The Octamer/Sox regulatory elements were hypermethylated in these genes. In addition, we found that GS cells express little Sox2 protein and low Oct3/4 protein despite abundant expression of their transcripts.ConclusionOur results suggest that DNA hypermethylation and transcriptional repression of a small set of ECATs, together with post-transcriptional repression of Oct3/4 and Sox2, contribute to the loss of pluripotency in male germ cells. |
| Databáze: | OpenAIRE |
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