Effect of Arachidonic Acid on the Rate of Oxygen Consumption in Isolated Cardiomyocytes from Intact Rats and Animals with Ischemic or Diabetic Injury to the Heart
| Autor: | Afanas'ev Sa, T. V. Kutsykova, Sergey V. Popov, Margarita V. Egorova |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Myocardial Infarction Infarction chemistry.chemical_element Oxygen General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental chemistry.chemical_compound Oxygen Consumption Internal medicine Diabetes mellitus Respiration medicine Animals Myocytes Cardiac Myocardial infarction Rats Wistar Incubation Arachidonic Acid Myocardium General Medicine Oxygenation medicine.disease Mitochondria Rats Endocrinology chemistry Biochemistry Arachidonic acid |
| Zdroj: | Bulletin of experimental biology and medicine. 160(2) |
| ISSN: | 1573-8221 |
| Popis: | We studied the rate of oxygen consumption by isolated cardiomyocytes from intact rats and animals with experimental myocardial infarction or streptozotocin-induced diabetes mellitus. The measurements were performed in standard incubation medium under various conditions of oxygenation and after addition of arachidonic acid (20 μmol/liter). Under normoxic conditions, arachidonic acid improves respiration of cardiomyocytes from intact animals, but reduces this parameter in cells isolated from animals with pathologies. The intensity of O2 consumption by cardiomyocytes from intact rats and animals with pathologies was shown to decrease during hypoxia. Addition of arachidonic acid aggravated inhibition of respiration for cardiomyocytes from intact rats and specimens with myocardial infarction, but had no effect in diabetes mellitus. The effect of arachidonic acid on oxygen consumption rate is probably mediated by a nonspecific mechanism realized at the mitochondrial level. |
| Databáze: | OpenAIRE |
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