Protection of OK-432, aStreptococcus pyogenesPreparation, against Lethal Infection of Mice with Herpes Simplex Virus
| Autor: | Hisao Endo, Yusuke Harada, Masahiko Fujii, Kyogo Ito, Motoo Saito, Hiroshi Irie, Yoshinobu Shimizu, Yutaka Sugawara, Katsuo Kumagai, Kimura Takao, Eri Kurokawa |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Cellular immunity T-Lymphocytes Immunology Dose-Response Relationship Immunologic Mice Nude Spleen Biology medicine.disease_cause Microbiology Peripheral blood mononuclear cell Antibodies Virus Herpesviridae Picibanil Mice Antigen Virology medicine Animals Simplexvirus Antigens Bacterial Biological Products Mice Inbred BALB C Mice Inbred C3H Herpes Simplex Macrophage Activation Silicon Dioxide Killer Cells Natural Herpes simplex virus medicine.anatomical_structure Streptococcus pyogenes Injections Intraperitoneal |
| Zdroj: | Microbiology and Immunology. 33:467-477 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.1989.tb01996.x |
| Popis: | We have studied the protective effect of OK-432, a biological response modifier (BRM) of Streptococcus pyogenes origin, on the lethal infection of mice with herpes simplex virus (HSV)-1. A single intraperitoneal (i.p.) injection of more than 10 micrograms of OK-432, when given at least two days before the infection, gave a marked effect yielding nearly 100% protection against ordinarily lethal infection. The protection was independent of the amount of infected virus inoculated. When given after the infection, the agent even at the maximal dose (100 micrograms), produced only a marginal effect. A single i.p. administration of OK-432 augmented the natural killer (NK) activity of peritoneal exudate cells and spleen mononuclear cells in mice 2 to 3 days after injection of OK-432, coinciding with the times when it induced a survival effect on HSV-infection. Treating OK-432-treated mice with a combination of an anti-macrophage agent, silica, and an anti-NK cell agent, anti-asialo GM1 serum, before infection diminished the antiviral effect of OK-432. The OK-432 protection against HSV infection was also markedly diminished in athymic nude mice. Thus, the protective effect of OK-432 on lethal HSV infection seems to be based on the activation of NK cells, macrophages, and T lymphocytes. |
| Databáze: | OpenAIRE |
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