A Combination of Recombinant HA1-and Nucleoprotein-Based Chitosan Nanoparticles Induces Early and Potent Immune Responses Against the H9N2 Influenza Virus

Autor:	Shahla Shahsavandi, Mohammad Majid Ebrahimi, Fatemeh Fotouhi, Majid Tebianian
Rok vydání:	2022
Předmět:	Chitosan Immunology Immunity Antibodies Viral Nucleoproteins Vaccines Inactivated Influenza Vaccines Influenza in Birds Virology Influenza Human Influenza A Virus H9N2 Subtype Animals Humans Nanoparticles Molecular Medicine Chickens
Zdroj:	Viral Immunology. 35:365-374
ISSN:	1557-8976 0882-8245
DOI:	10.1089/vim.2021.0207
Popis:	The effectiveness of inactivated H9N2 influenza vaccines is doubtful due to changes in antigenic regions of the virus hemagglutinin (HA) protein. One strategy for the development of the efficacious vaccine is the use of nanoparticles that display more immunogenic regions of the influenza virus. In this study, chitosan (CS)-based nanoparticles were developed as a delivery system for intranasal immunization using recombinant H9N2 virus HA1 and nucleoprotein (NP), for the induction of humoral and cellular responses. CS-HA1 and CS-NP nanoparticles were prepared by the ionic gelation method and characterized for their physicochemical properties and shape. The immunogenicity and the protective efficacy were evaluated by measuring antibody titers, T cell proliferation response, CD4
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23fc33a9dd9998b83d8c334e9367c0cb https://doi.org/10.1089/vim.2021.0207 Zobrazit plný text záznamu