High-phosphorus diet maximizes and low-dose calcitriol attenuates skeletal muscle changes in long-term uremic rats
| Autor: | Carmen Pineda, José-Luis L. Rivero, Luz M. Acevedo, Alan Peralta-Ramírez, Mariano Rodriguez, Escolastico Aguilera-Tejero, Verónica Chamizo, Ignacio González López |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Calcitriol Physiology 030232 urology & nephrology chemistry.chemical_element Parathyroid hormone Calcium 03 medical and health sciences 0302 clinical medicine Physiology (medical) Internal medicine Myosin medicine Animals Muscle Skeletal Uremia business.industry Skeletal muscle Phosphorus medicine.disease Diet Rats Cross-Sectional Studies 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Parathyroid Hormone Kidney Failure Chronic Female Secondary hyperparathyroidism business Kidney disease medicine.drug |
| Zdroj: | Journal of Applied Physiology. 120:1059-1069 |
| ISSN: | 1522-1601 8750-7587 |
| Popis: | Although disorders of mineral metabolism and skeletal muscle are common in chronic kidney disease (CKD), their potential relationship remains unexplored. Elevations in plasma phosphate, parathyroid hormone, and fibroblastic growth factor 23 together with decreased calcitriol levels are common features of CKD. High-phosphate intake is a major contributor to progression of CKD. This study was primarily aimed to determine the influence of high-phosphate intake on muscle and to investigate whether calcitriol supplementation counteracts negative skeletal muscle changes associated with long-term uremia. Proportions and metabolic and morphological features of myosin-based muscle fiber types were assessed in the slow-twitch soleus and the fast-twitch tibialis cranialis muscles of uremic rats (5/6 nephrectomy, Nx) and compared with sham-operated (So) controls. Three groups of Nx rats received either a standard diet (0.6% phosphorus, Nx-Sd), or a high-phosphorus diet (0.9% phosphorus, Nx-Pho), or a high-phosphorus diet plus calcitriol (10 ng/kg 3 day/wk ip, Nx-Pho + Cal) for 12 wk. Two groups of So rats received either a standard diet or a high-phosphorus diet (So-Pho) over the same period. A multivariate analysis encompassing all fiber-type characteristics indicated that Nx-Pho + Cal rats displayed skeletal muscle phenotypes intermediate between Nx-Pho and So-Pho rats and that uremia-induced skeletal muscle changes were of greater magnitude in Nx-Pho than in Nx-Sd rats. In uremic rats, treatment with calcitriol preserved fiber-type composition, cross-sectional size, myonuclear domain size, oxidative capacity, and capillarity of muscle fibers. These data demonstrate that a high-phosphorus diet potentiates and low-dose calcitriol attenuates adverse skeletal muscle changes in long-term uremic rats. |
| Databáze: | OpenAIRE |
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