Complete monitoring of the purification of the plasma membrane from rabbit skeletal muscle
| Autor: | G. Serratrice, Alain Lombet, S. Maroux, C. Desnuelle, D. Liot |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Sucrose
ATPase Biophysics Tetrodotoxin Cell Fractionation Biochemistry Ouabain chemistry.chemical_compound Cell surface receptor Centrifugation Density Gradient medicine Animals Molecular Biology Chromatography biology Muscles Cell Membrane Skeletal muscle Cell Biology Phosphoric Monoester Hydrolases Receptor Insulin Insulin receptor Membrane medicine.anatomical_structure chemistry biology.protein Rabbits medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 112:521-527 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(83)91496-1 |
| Popis: | A fast and reproducible purification procedure for rabbit skeletal muscle plasma membrane is described. Each step was monitored by determination of tetrodotoxin, ouabain and insulin receptors. A ouabain-sensitive K + -stimulated and a Ca 2+ -dependent phosphatases, probably identical to, respectively the (Na + K + ) and Ca 2+ -ATPases, were also evaluated. All plasma membrane receptors and the ouabain-sensitive activity accumulated in the ligatest fraction separated by sucrose gradient centrifugation (peak at 18% sucrose; purification from crude homogenate, 30-fold). |
| Databáze: | OpenAIRE |
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