Procollagen-Like Protein as a Molecular Target in the Treatment of Primary Brain Tumor
| Autor: | Howard Landy, Jenny Xu, Rong Xu, Lynn G. Feun, Medhi Wangpaichitr, Chun Jing Wu, Niramol Savaraj |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Messenger RNA
lcsh:T Chemistry cDNA library medicine.drug_class lcsh:R Short Report lcsh:Medicine General Medicine medicine.disease Monoclonal antibody lcsh:Technology Molecular biology Calcitriol receptor General Biochemistry Genetics and Molecular Biology Procollagen peptidase Cell culture Putative gene Glioma medicine lcsh:Q lcsh:Science General Environmental Science |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 2, Pp 125-126 (2002) |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | METHODS. cDNA library was obtained in one glioma cell line which highly expressed this protein and screening was performed using VDR monoclonal antibody. This partial cDNA was then subcloned into pcDNA3.1 and sequencing was performed. This partial cDNA sequence also was used as a probe to identify mRNA, which encodes for this putative gene. To characterize this 220-Kd protein, we have used five cell lines as follows: Glioma 1, U118, SW1783, which possess variable amount of 220-Kd protein, and U-373 and T-98G which lack this 220-Kd protein. |
| Databáze: | OpenAIRE |
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