Plasma, Cerebrospinal Fluid, and Brain Distribution of14C-Melatonin in Rat: A Biochemical and Autoradiographic Study
| Autor: | P.A. Vitte, C. Harthe, Bruno Claustrat, P. Bobillier, P. Lestage |
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| Rok vydání: | 1988 |
| Předmět: |
Male
medicine.medical_specialty Blood–brain barrier Endocrinology Cerebrospinal fluid Internal medicine Blood plasma medicine Animals Tissue Distribution Carbon Radioisotopes Pretectal area Melatonin Volume of distribution Chromatography Chemistry Brain Rats Inbred Strains Rats Kinetics medicine.anatomical_structure Blood-Brain Barrier Hypothalamus Autoradiography Biological half-life Nucleus Half-Life |
| Zdroj: | Journal of Pineal Research. 5:437-453 |
| ISSN: | 1600-079X 0742-3098 |
| DOI: | 10.1111/j.1600-079x.1988.tb00787.x |
| Popis: | The distribution of 14C-Melatonin (14C-MT) after systemic injection was studied in the plasma, cerebrospinal fluid (CSF), and brain of rats. Chromatographic analysis (thin-layer chromatography and high-performance liquid chromatography) indicated that the radioactivity from biological samples taken at various times following the injection of label was mainly associated with 14C-MT. Computer analysis of plasma 14C-MT kinetics showed a three-compartment system with half-lives of 0.21 +/- 0.05, 5.97 +/- 1.11, and 47.52 +/- 8.86 min. The volume of distribution and the clearance were 1,736 +/- 349 ml.kg-1 and 25.1 +/- 1.7 ml.min-1.kg-1 respectively. The entry of 14C-MT into the CSF was rapid and reached a maximum at 5 min. The decay followed a two-compartment system with half-lives of 16.5 +/- 2.9 and 47.3 +/- 8.6 min. The CSF/plasma concentration ratio was 0.38 at the steady state (30 min). At 2 min the level of 14C-MT in the brain was 3.8 higher than in the CSF. Representative autoradiograms revealed an heterogeneous localization of 14C-MT in the grey matter. The highest regional values, as evaluated by the permeability area product technique, were found in cortex, thalamic nuclei, medial geniculate nucleus, anterior pretectal area, paraventricular nucleus of the hypothalamus, choroid plexuses, and bulb-pons. Thirty minutesmore » later 14C-MT was still detected in most of the brain regions analyzed. These results point to a low but rapid penetration of circulating MT into the brain and the CSF. The heterogeneous distribution and the partial retention of 14C-MT in the brain are compatible with the hypothesis of a central action of this hormone mediated via binding sites.« less |
| Databáze: | OpenAIRE |
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