Effect of protease inhibitor-containing regimens on lymphocyte multidrug resistance transporter expression
| Autor: | Saye Khoo, Mark Dalton, J. Ford, E. R. Meaden, Philippa Newton, Ian S. Williams, David Back, Patrick G. Hoggard |
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| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Adult CD4-Positive T-Lymphocytes Male viruses CD4-CD8 Ratio HIV Infections Biology Pharmacology CD8-Positive T-Lymphocytes In Vitro Techniques Amprenavir immune system diseases In vivo Indinavir medicine Humans Pharmacology (medical) Protease inhibitor (pharmacology) ATP Binding Cassette Transporter Subfamily B Member 1 Lymphocyte Count Lymphocytes virus diseases Lopinavir HIV Protease Inhibitors biochemical phenomena metabolism and nutrition Middle Aged Flow Cytometry Virology Infectious Diseases Nelfinavir Ritonavir Female Multidrug Resistance-Associated Proteins Saquinavir medicine.drug |
| Zdroj: | The Journal of antimicrobial chemotherapy. 52(3) |
| ISSN: | 0305-7453 |
| Popis: | BACKGROUND: Increased expression of multidrug resistance transporters, such as P-glycoprotein (P-gp), has been suggested as a potential mechanism for decreased protease inhibitor (PI) availability at certain intracellular sites and tissue compartments. OBJECTIVES: To investigate the effect of PIs on the surface lymphocyte expression of P-gp in vitro and in vivo. PATIENTS AND METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy subjects (n = 15) and incubated (72 h) with 10 microM of each PI studied (saquinavir, ritonavir, nelfinavir, indinavir, amprenavir and lopinavir), or dimethyl sulphoxide (DMSO) control. PBMCs were also isolated from HIV-infected subjects (n = 50; viral load |
| Databáze: | OpenAIRE |
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