Infection of apical dentin and root-end cavity disinfection
| Autor: | Andrew McNaughton, Abdul Aziz, Nicholas P. Chandler, Geoffrey R. Tompkins, Jonathan W. Leichter, C. H. J. Hauman |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Materials science Ultrasonic Therapy Cetrimide Smear layer Colony Count Microbial Dentistry Enterococcus faecalis Dentinal Fluid stomatognathic system Tooth Apex Dentin medicine Humans Cementum General Dentistry Edetic Acid Dental Cementum Microscopy Confocal biology Root Canal Irrigants business.industry Chlorhexidine Penetration (firestop) biology.organism_classification Endodontics Disinfection stomatognathic diseases medicine.anatomical_structure Smear Layer Lasers Semiconductor business Root Canal Preparation medicine.drug |
| Zdroj: | Journal of endodontics. 38(10) |
| ISSN: | 1878-3554 |
| Popis: | Introduction The purpose of this study was to assess Enterococcus faecalis penetration into the dentin of the apical 3 mm and bacterial death after the application of either chlorhexidine or laser to root-end cavities. Methods Root canals of 60 single-rooted teeth were prepared. In part 1, cementum was removed semicircumferentially from 21 roots, and the smear layer was removed from 15 roots using 17% EDTA/cetrimide. Teeth were inoculated and incubated with E. faecalis for 10 days, rinsed, and live/dead stained. The effect of cementum and smear on bacterial penetration was assessed by confocal laser scanning microscopy (CLSM). In part 2, 39 teeth had root ends resected and cavities ultrasonically prepared. Inoculated roots were assigned to 1 of the following 3 groups: (1) root-end cavities irrigated with 0.2 % chlorhexidine, (2) root-end cavities irradiated with a laser for 20 seconds at 1.5 W, or (3) root-end cavities that received no treatment. Roots were live/dead stained, sectioned, and examined by CLSM. The depth of the bacterial penetration and bacterial survival were compared using the Mann-Whitney U test. Results The presence of a smear layer and/or cementum did not significantly affect bacterial penetration. In root-end cavities, chlorhexidine was more effective than laser ( P Conclusions E. faecalis invaded the entire width of dentin in the apical 3 mm irrespective of the smear layer and/or cementum. Chlorhexidine was more effective than laser in disinfecting root-end cavities. |
| Databáze: | OpenAIRE |
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