Roles of regulatory T cells in the pathogenesis of pediatric aplastic anemia
| Autor: | Bi-Hong Zhang, Qi-Hui Chen, Chun Chen, Su Liu, Hongman Xue, Junbin Huang, Jian Wang, Lele Hou, Shaofen Lin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
STAT3 Transcription Factor Bone Marrow Cells T-Lymphocytes Regulatory Pathogenesis 03 medical and health sciences 0302 clinical medicine Medicine Humans RNA Messenger Aplastic anemia skin and connective tissue diseases Child business.industry Anemia Aplastic Forkhead Transcription Factors Hematology medicine.disease Neoplasm Proteins Oncology 030220 oncology & carcinogenesis Child Preschool Pediatrics Perinatology and Child Health Immunology Female sense organs business T-Box Domain Proteins 030215 immunology |
| Zdroj: | Pediatric hematology and oncology. 36(4) |
| ISSN: | 1521-0669 |
| Popis: | The pathogenesis of aplastic anemia (AA) in children is not clear. This study was conducted to investigate the changes in the proportion and function of regulatory T cells (Tregs) in pediatric AA. The proportion of Tregs, mRNA levels of transcription factors, and concentrations of cytokines were measured by flow cytometry, reverse transcription-PCR, and enzyme-linked immunosorbent assay, respectively. Tregs were co-cultured with effector T cells (Teff) to evaluate the function of Tregs. The proportion of Tregs after immunosuppressive therapy (IST) in pediatric AA was monitored dynamically. Compared to the control, the proportions of Tregs in peripheral blood and bone marrow lymphocytes of the untreated AA group were lower (1.31% ± 0.73% vs. 3.16% ± 0.92%, 1.49% ± 0.81% vs. 3.06% ± 0.82%, respectively |
| Databáze: | OpenAIRE |
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