Screening of Lipids for Early Triage and Dose Estimation after Acute Radiation Exposure in Rat Plasma Based on Targeted Lipidomics Analysis
| Autor: | Tian-Jing Cai, Ke-Hui Liu, Xue Lu, Shuang Li, Cong Xi, Hua Zhao, Mei Tian, Qing-Jie Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Biochemistry 03 medical and health sciences Plasma Biodosimetry Internal medicine Dose estimation Lipidomics medicine Animals Acute radiation exposure 030102 biochemistry & molecular biology Receiver operating characteristic business.industry Selected reaction monitoring General Chemistry Total body irradiation Radiation Exposure Triage Lipids Rats 030104 developmental biology business |
| Zdroj: | Journal of proteome research. 20(1) |
| ISSN: | 1535-3907 |
| Popis: | Rapid early triage and dose estimation is vital for limited medical resource allocation and treatment of a large number of the wounded after radiological accidents. Lipidomics has been utilized to delineate biofluid lipid signatures after irradiation. Here, high-coverage targeted lipidomics was employed to screen radiosensitive lipids after 0, 1, 2, 3, 5, and 8 Gy total body irradiation at 4, 24, and 72 h postirradiation in rat plasma. Ultra-performance liquid chromatography-tandem mass spectrometry with a multiple reaction monitoring method was utilized. In total, 416 individual lipids from 18 major classes were quantified and those biomarkers altered in a dose-dependent manner constituted panel A-panel D. Receiver operator characteristic curve analysis using combined lipids showed good to excellent sensitivity and specificity in triaging different radiation exposure levels (area under curve = 0.814-1.000). The equations for dose estimation were established by stepwise regression analysis for three time points. A novel strategy for radiation early triage and dose estimation was first established and validated using panels of lipids. Our study suggests that it is feasible to acquire quantitative lipid biomarker panels using targeted lipidomics platforms for rapid, high-throughput triage, which can provide further insights in developing lipidomics strategies for radiation biodosimetry in humans. |
| Databáze: | OpenAIRE |
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