Benchtop plasma treatment of titanium surfaces enhances cell response
| Autor: | Barbara D. Boyan, Zvi Schwartz, Kyla B. Bosh, Michael B. Berger, D. Joshua Cohen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Surface Properties medicine.medical_treatment chemistry.chemical_element 02 engineering and technology Dielectric barrier discharge Article Plasma 03 medical and health sciences 0302 clinical medicine Adsorption medicine General Materials Science Dental implant General Dentistry Dental Implants Titanium Osteoblasts Cell Differentiation Osteoblast 030206 dentistry 021001 nanoscience & nanotechnology Surface energy medicine.anatomical_structure chemistry Mechanics of Materials Wetting Implant 0210 nano-technology Biomedical engineering |
| Zdroj: | Dent Mater |
| ISSN: | 0109-5641 |
| DOI: | 10.1016/j.dental.2021.01.026 |
| Popis: | Objective Modifications to implant surface properties, including topography, chemistry, and wettability, alter immune response, osteoblast differentiation of bone marrow stromal cells (MSCs), and implant integration in vivo. Dielectric barrier discharge (DBD) plasma treatment has been used to sterilize surfaces and remove adsorbed carbon, improving wettability. However, unless it is used immediately prior to placement, ambient atmospheric hydrocarbons rapidly adhere to the surface, thereby reducing its hydrophilicity. Moreover, this method is not practical in many clinical settings. The aim of this study was to evaluate the effectiveness of an on-site benchtop modification technique for implants at time of placement, consisting of a DBD plasma that is used to sterilize implants that are pre-packaged in a vacuum. Effects of the plasma-treatment on implant surface properties and cellular response of MSCs and osteoblasts were assessed in vitro. Methods Titanium-aluminum-vanadium implant surfaces were grit-blasted (GB) or grit-blasted and acid-etched (AE), and packaged under vacuum. AE surfaces were also plasma-treated using the benchtop device (GB + AE) and then removed from the vacuum. GB surface morphology was altered with AE but AE microroughness was not changed with the plasma-treatment. Plasma-treatment increased the surface wettability, but did not alter surface atomic concentrations of titanium, oxygen, or carbon. Results MSCs and osteoblast-like cells (MG63 s) produced increased concentrations of osteocalcin, osteopontin, and osteoprotegerin after plasma-treatment of AE surfaces compared to non-plasma-treated AE surfaces; production of IL6 was reduced and IL10 was. Aging GB + AE surfaces for 7 days after plasma-treatment but still in the vacuum environment reduced the effectiveness of plasma on cellular response. Significance Overall, these data suggest that application of benchtop plasma at the time of implant placement can alter the surface free energy of an implant surface without modifying surface chemical composition and enhance the differentiation and activity of MSCs and osteoblasts that are in contact with these implant surfaces. |
| Databáze: | OpenAIRE |
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