Polysorbate 80 UV/vis spectral and chromatographic characteristics – defining boundary conditions for use of the surfactant in dissolution analysis
| Autor: | W. Peter Wuelfing, Robert A. Reed, Amy Harman, Allen C. Templeton, Kathryn Kosuda, Mark D. Mowery |
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| Rok vydání: | 2006 |
| Předmět: |
Polysorbate
Chromatography medicine.diagnostic_test Elution Clinical Biochemistry Polysorbates Pharmaceutical Science Reversed-phase chromatography Analytical Chemistry Surface-Active Agents chemistry.chemical_compound Ultraviolet visible spectroscopy chemistry Pulmonary surfactant Spectrophotometry Drug Discovery medicine Spectrophotometry Ultraviolet Solubility Dissolution Chromatography High Pressure Liquid Spectroscopy |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 41:774-782 |
| ISSN: | 0731-7085 |
| Popis: | Polysorbate 80 is used in the pharmaceutical industry as an additive to enhance the solubility of non-polar compounds in formulation design and during dissolution analysis. In this paper we present the spectroscopic and chromatographic characteristics for a series of commercially available sources of this non-ionic surfactant. The large UV/vis absorbance and broad chromatographic elution of Polysorbate 80 often makes it difficult to accurately quantitate pharmaceutically active compounds in solutions where the surfactant is present. Boundary conditions have been established where analytical interferences can be avoided in spectrophotometric analysis by choice of analysis wavelength and solution concentrations. Chromatographic method development is also presented enabling the removal of Polysorbate interference in instances where spectroscopic interference is too great. |
| Databáze: | OpenAIRE |
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