Molecular cloning of the gene for the allatostatin family of neuropeptides from the cockroach Diploptera punctata
| Autor: | B C Donly, William G. Bendena, Stephen S. Tobe, Qi Ding |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
ved/biology.organism_classification_rank.species Cockroaches Genes Insect Biology Molecular cloning Polymerase Chain Reaction digestive system fluids and secretions Complementary DNA Gene expression polycyclic compounds Animals Amino Acid Sequence Cloning Molecular Protein Precursors Gene DNA Primers Southern blot Multidisciplinary Base Sequence Sequence Homology Amino Acid ved/biology Neuropeptides Diploptera punctata virus diseases Brain Allatostatin DNA Molecular biology digestive system diseases Blotting Southern Multigene Family Female Corpus allatum Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:8807-8811 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.90.19.8807 |
| Popis: | Allatostatins (ASTs) are insect neuropeptides that inhibit juvenile hormone biosynthesis by the corpora allata. We have isolated a cDNA from the cockroach Diploptera punctata that encodes a 41.5-kDa precursor polypeptide containing the AST family of peptides. Translation of the cDNA revealed a 370-amino acid pre-pro-peptide consisting of 13 AST-type peptides and appropriate processing sites for endoproteolytic cleavage and amidation. The 13 potential AST sequences are characterized by the C-terminal AST corestructure Phe-Gly-Leu-NH2, with only one exception. Separating the clustered ASTs in the precursor, three acidic spacer regions are found. Contained within the largest of these are two potentially related peptides that may also be processed. Southern blot analysis revealed the presence of a single copy of the AST gene per haploid genome, as well as the probability that the gene may be present in at least two allelic forms. In situ hybridization indicated the AST-encoding gene is expressed in neurosecretory cells of D. punctata brain. |
| Databáze: | OpenAIRE |
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