A molecular roadmap for the emergence of early-embryonic-like cells in culture
| Autor: | Arnaud Kopp, Takashi Ishiuchi, Audrey Penning, Diego Rodriguez-Terrones, Laurent Brino, Xavier Gaume, Maria-Elena Torres-Padilla, Amélie Weiss, Kai Kruse, Juan Jm Vaquerizas |
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| Přispěvatelé: | univOAK, Archive ouverte, Helmholtz Zentrum München = German Research Center for Environmental Health, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Kyushu University, Max Planck Institute for Molecular Biomedicine, Max-Planck-Gesellschaft, Ludwig-Maximilians-Universität München (LMU) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cellular differentiation Population [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Article Cell Line Epigenesis Genetic Transcriptome Mice 03 medical and health sciences Single-cell analysis Genetics Animals Epigenetics education [SDV.BC] Life Sciences [q-bio]/Cellular Biology Cells Cultured Embryonic Stem Cells education.field_of_study Totipotent Gene Expression Regulation Developmental Cell Differentiation Embryonic stem cell Cell biology 030104 developmental biology Cell culture Single-Cell Analysis Transcription Factors |
| Zdroj: | Nat. Genet. 50, 106-119 (2017) Nature Genetics Nature Genetics, 2017, 50 (1), pp.106-119. ⟨10.1038/s41588-017-0016-5⟩ Nature genetics |
| ISSN: | 1546-1718 1061-4036 |
| DOI: | 10.1038/s41588-017-0016-5 |
| Popis: | Unlike pluripotent cells, which generate only embryonic tissues, totipotent cells can generate a full organism, including extra-embryonic tissues. A rare population of cells resembling 2-cell-stage embryos arises in pluripotent embryonic stem (ES) cell cultures. These 2-cell-like cells display molecular features of totipotency and broader developmental plasticity. However, their specific nature and the process through which they arise remain outstanding questions. Here we identified intermediate cellular states and molecular determinants during the emergence of 2-cell-like cells. By deploying a quantitative single-cell expression approach, we identified an intermediate population characterized by expression of the transcription factor ZSCAN4 as a precursor of 2-cell-like cells. By using a small interfering RNA (siRNA) screen, we identified epigenetic regulators of 2-cell-like cell emergence, including the non-canonical PRC1 complex PRC1.6 and the EP400-TIP60 complex. Our data shed light on the mechanisms that underlie exit from the ES cell state toward the formation of early-embryonic-like cells in culture and identify key epigenetic pathways that promote this transition. |
| Databáze: | OpenAIRE |
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