Differential Homeostasis of Sessile and Pendant Epithelium Reconstituted in a 3D-Printed 'GeminiChip'
| Autor: | Shlomo Magdassi, Chwee Teck Lim, Xiaodong Chen, Benhui Hu, Changjin Wan, Zhuyun Li, Pingqiang Cai, Yun-Long Wu, Ying Jiang, Eugene V. Makeyev, Luying Wang, Ela Sachyani Keneth, Shutao Wang |
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| Přispěvatelé: | School of Materials Science & Engineering, Innovative Center for Flexible Devices (iFLEX) |
| Rok vydání: | 2019 |
| Předmět: |
RHOA
Materials science Cell Context (language use) tumor spheroids 02 engineering and technology 010402 general chemistry 01 natural sciences Epithelium Transcriptome Mechanobiology Downregulation and upregulation medicine Homeostasis General Materials Science Mechanical Phenomena organ-on-a-chip biology Mechanical Engineering Spheroid Biological sciences [Science] mechanobiology 021001 nanoscience & nanotechnology Epithelial Homeostasis 0104 chemical sciences Cell biology Biomechanical Phenomena 3D Printing medicine.anatomical_structure Mechanics of Materials Printing Three-Dimensional biology.protein Stress Mechanical 0210 nano-technology |
| Zdroj: | Cai, P, Li, Z, Keneth, E S, Wang, L, Wan, C, Jiang, Y, Hu, B, Wu, Y L, Wang, S, Lim, C T, Makeyev, E V, Magdassi, S & Chen, X 2019, ' Differential Homeostasis of Sessile and Pendant Epithelium Reconstituted in a 3D-Printed “GeminiChip” ', ADVANCED MATERIALS, vol. 31, no. 28, 1900514 . https://doi.org/10.1002/adma.201900514 |
| ISSN: | 1521-4095 |
| DOI: | 10.1002/adma.201900514 |
| Popis: | Local mechanical cues can affect crucial fate decisions of living cells. Transepithelial stress has been discussed in the context of epithelial monolayers, but the lack of appropriate experimental systems leads current studies to approximate it simply as an in‐plane stress. To evaluate possible contribution of force vectors acting in other directions, double epithelium in a 3D‐printed “GeminiChip” containing a sessile and a pendant channel is reconstituted. Intriguingly, the sessile epithelia is prone to apoptotic cell extrusion upon crowding, whereas the pendant counterpart favors live cell delamination. Transcriptome analyses show upregulation of RhoA, BMP2, and hypoxia‐signaling genes in the pendant epithelium, consistent with the onset of an epithelial–mesenchymal transition program. HepG2 microtumor spheroids also display differential spreading patterns in the sessile and pendant configuration. Using this multilayered GeminiChip, these results uncover a progressive yet critical role of perpendicular force vectors in collective cell behaviors and point at fundamental importance of these forces in the biology of cancer. NRF (Natl Research Foundation, S’pore) Accepted version |
| Databáze: | OpenAIRE |
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