Immunoglobulin gene rearrangement in plasma cell dyscrasias: detection of small clonal cell populations in peripheral blood and bone marrow
| Autor: | Kurt Grünewald, J. Drach, Falko Fend, Florian Umlauft, Andrea Schwaiger, K. Weyrer |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Plasma cell dyscrasia Paraproteinemias Bone Neoplasms Plasma cell Monoclonal Gammopathy of Undetermined Significance Flow cytometry Immunophenotyping Bone Marrow Precursor cell medicine Humans Gene Rearrangement B-Lymphocyte Multiple myeloma Aged Blood Cells biology medicine.diagnostic_test Genes Immunoglobulin Hematology DNA Neoplasm Middle Aged medicine.disease Clone Cells Blotting Southern medicine.anatomical_structure Myeloma Proteins Oncology Monoclonal biology.protein Neoplastic Stem Cells Female Bone marrow Antibody Multiple Myeloma Plasmacytoma |
| Zdroj: | Leukemialymphoma. 10(3) |
| ISSN: | 1042-8194 |
| Popis: | The bone marrow (BM) and peripheral blood (PB) samples of 71 patients with plasma cell dyscrasias were analysed by the Southern blot technique for the presence of clonal immunoglobulin (Ig) gene rearrangements. 53% of BM samples examined were archival material such as air dried BM slides or frozen trephine biopsies. The results were related to bone marrow plasmacytosis as determined by cytology and flow cytometry, and other clinical parameters. Clonal Ig gene rearrangements were found in BM samples of 45 (83%) of 54 MM patients and in 3 of 6 patients with monoclonal gammopathy of unknown significance (MGUS). Clonal cell populations in the PB were detected in 11 (30%) of 37 examined MM patients, but in none of the patients with MGUS or solitary plasmacytoma of bone. PB involvement was associated with progressive disease. Circulating monoclonal cells were significantly associated with higher M-protein levels (p 0.05). Thus, circulating clonal precursor cells are encountered more frequently in active MM. |
| Databáze: | OpenAIRE |
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