Label-free multimodal nonlinear optical microscopy for biomedical applications
| Autor: | Fu Jen Kao, Viktor V. Nikolaev, Sindhoora K M, N. A. Krivova, Nirmal Mazumder, Yury V. Kistenev, Spandana K U, Hala Zuhayri, Guan Yu Zhuo |
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| Rok vydání: | 2021 |
| Předmět: |
010302 applied physics
неинвазивная молекулярная визуализация Fluorescence-lifetime imaging microscopy Microscope Materials science business.industry Physics::Optics General Physics and Astronomy Context (language use) 02 engineering and technology 021001 nanoscience & nanotechnology Laser 01 natural sciences law.invention law 0103 physical sciences Microscopy Femtosecond Optoelectronics Molecular imaging 0210 nano-technology business биомедицинские приложения нелинейная оптическая микроскопия Photonic-crystal fiber |
| Zdroj: | Journal of applied physics. 2021. Vol. 129, № 21. P. 214901-214901-14 |
| ISSN: | 1089-7550 0021-8979 |
| DOI: | 10.1063/5.0036341 |
| Popis: | This paper addresses the application of multimodal nonlinear optical (MNLO) microscopy to clinical research within the context of label-free non-invasive molecular imaging. Here, a compact MNLO microscope based on a laser scanning microscope, a femtosecond laser, a time-correlated single-photon counting system, and a photonic crystal fiber are introduced for biomedical applications. By integrating two-photon fluorescence, two-photon fluorescence lifetime imaging, second-harmonic generation, and coherent anti-Stokes Raman scattering microscopy, the proposed scheme provides profound insights into the physicochemical properties related to 3D molecular orientation distribution, inter- and intra-molecular interactions, and disease progression in biological systems and organs. The high peak power and the low average intensity of near-infrared laser pulses allow for deep-penetration imaging without compromising sample vitality. Linking nonlinear optical phenomena with time/spectral/polarization-resolved imaging also makes it possible to obtain multidimensional information to address complex biomedical questions. |
| Databáze: | OpenAIRE |
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