Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells
| Autor: | Magalie Rocheville, Enrico Gratton, Ujendra Kumar, Yogesh C. Patel, Aruna Rani, Don C. Lamb, Shutish C. Patel, John Eid, Theodore L. Hazlett, Michael P. Grant, Ramesh C. Patel |
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| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
Multidisciplinary
Chemistry Somatostatin receptor Ligand binding assay Plasma protein binding CHO Cells Biological Sciences Ligand (biochemistry) Ligands Förster resonance energy transfer Spectrometry Fluorescence Biochemistry Cricetinae Biophysics Animals Somatostatin receptor 1 Receptors Somatostatin Receptor Oligopeptides G protein-coupled receptor Fluorescent Dyes Protein Binding |
| Zdroj: | Patel, RC; Kumar, U; Lamb, DC; Eid, JS; Rocheville, M; Grant, M; et al.(2002). Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 99(5), 3294-3299. doi: 10.1073/pnas.042705099. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/3s94v8tf |
| Popis: | Heptahelical receptors (HHRs) are generally thought to function as monomeric entities. Several HHRs such as somatostatin receptors (SSTRs), however, form homo- and heterooligomers when activated by ligand binding. By using dual fluorescent ligands simultaneously applied to live cells monotransfected with SSTR5 (R5) or SSTR1 (R1), or cotransfected with R5 and R1, we have analyzed the ligand receptor stoichiometry and aggregation states for the three receptor systems by fluorescence resonance energy transfer and fluorescence correlation spectroscopy. Both homo- and heterooligomeric receptors are occupied by two ligand molecules. We find that monomeric, homooligomeric, and heterooligomeric receptor species occur in the same cell cotransfected with two SSTRs, and that oligomerization of SSTRs is regulated by ligand binding by a selective process that is restricted to some (R5) but not other (R1) SSTR subtypes. We propose that induction by ligand of different oligomeric states of SSTRs represents a unique mechanism for generating signaling specificity not only within the SSTR family but more generally in the HHR family. |
| Databáze: | OpenAIRE |
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