Enzymes in uncultured amniotic fluid cells
| Autor: | R.G. Sutcliffe, David J. H. Brock |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Glucose-6-phosphate isomerase
Erythrocytes Time Factors Clinical Biochemistry Dehydrogenase Punctures Urine Glucosephosphate Dehydrogenase Biology Biochemistry chemistry.chemical_compound Pregnancy Lactate dehydrogenase Humans Fluorometry Amnion chemistry.chemical_classification L-Lactate Dehydrogenase Amniotic fluid cells Phosphogluconate Dehydrogenase Biochemistry (medical) Glucose-6-Phosphate Isomerase General Medicine Metabolism Amniotic Fluid Molecular biology Isocitrate Dehydrogenase Enzyme Isocitrate dehydrogenase chemistry Spectrophotometry Female Metabolism Inborn Errors |
| Zdroj: | Clinica Chimica Acta. 31:363-365 |
| ISSN: | 0009-8981 |
| Popis: | The activities of five enzymes, isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphohexose isomerase and lactate dehydrogenase have been measured in amniotic fluid cells at different stages of pregnancy. With the exception of phosphohexose isomerase very low and highly variable values were found and it was necessary to use spectrofluorometry to detect activity. Contamination of samples by red cells was a frequent problem. The usefulness of enzyme measurements in uncultured amniotic fluid cells for the antenatal diagnosis of inborn errors of metabolism is discussed in the light of these experiences with undiluted urine. |
| Databáze: | OpenAIRE |
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