Advanced comparative cytogenetic analysis of X chromosomes in river buffalo, cattle, sheep, and human
Autor: | Angela Perucatti, Alexander Rebl, Leopoldo Iannuzzi, Alessandra Iannuzzi, Tom Goldammer, V. Genualdo, D. Di Berardino |
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Přispěvatelé: | Perucatti, A., Genualdo, V., Iannuzzi, A., Rebl, A., DI BERARDINO, Dino, Goldammer, T., Iannuzzi, Leopoldo |
Jazyk: | angličtina |
Rok vydání: | 2012 |
Předmět: |
Male
sheep Buffaloes Centromere Pseudoautosomal region Locus (genetics) Biology Y chromosome X chromosome FISH Y Chromosome Genetics Animals Humans human In Situ Hybridization Fluorescence Synteny Bacterial artificial chromosome Gene map Physical Chromosome Mapping comparative mapping cattle Cytogenetic Analysis Female river buffalo |
Zdroj: | Chromosome research 20 (2012): 413–425. doi:10.1007/s10577-012-9285-0. info:cnr-pdr/source/autori:Perucatti A; Genualdo V; Iannuzzi A; Rebl A; Di Berardino D; Goldammer T; Iannuzzi L./titolo:Advanced comparative cytogenetic analysis of X chromosomes in river buffalo, cattle, sheep, and human./doi:10.1007%2Fs10577-012-9285-0./rivista:Chromosome research/anno:2012/pagina_da:413/pagina_a:425/intervallo_pagine:413–425/volume:20 |
Popis: | Based on a recently generated comprehensive gene map for Ovis aries chromosome X (OARX) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (BAC) probes corresponding to these OARX loci to Bubalus bubalis (BBU) and Bos taurus (BTA) by comparative fluorescence in-situ hybridization (FISH) to improve cytogenetically the X chromosome maps in these species. Twenty-five added loci in BBUX and BTAX, respectively, contribute to a more detailed description of the cytogenetic organization of these chromosomes. Further seven loci were identified in OARX and two DNA probes were assigned to X and Y chromosomes in river buffalo, cattle, and sheep, respectively, and thus identified loci in the pseudoautosomal region. The additional assignments double the number of cytogenetic loci in BBUX and increase their number in BTAX and OARX. The larger quantity of cytogenetic anchors allows a more precise morphological comparison of bovid X chromosomes among each other and with the Homo sapiens (HSA) X chromosome. The anchor loci confirm and refine syntenic fragments in HSAX and identify several evolutionary breakpoints between the compared chromosomes. The cytogenetic assignments in BBUX, BTAX, and OARX represent useable anchors for the ongoing genome sequence assembly in Bovidae. |
Databáze: | OpenAIRE |
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