Genomic characterization of four novel bacteriophages infecting the clinical pathogen Klebsiella pneumoniae
| Autor: | Teunke van Rossum, Wenchen Song, Ana Rita Costa, Stefan Hagedoorn, Minfeng Xiao, Hielke Walinga, Pieter-Jan A. Haas, Daan F. van den Berg, Estrada Bonilla, Stan J. J. Brouns, Franklin L. Nobrega |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
phage therapy Phage therapy Klebsiella pneumoniae viruses medicine.medical_treatment AcademicSubjects/MED00774 Genome Viral comparative genomics Biology Genome Resource Article: Genomes Explored Bacteriophage Viral Proteins bacteriophage Genetics medicine Bacteriophages Molecular Biology Phylogeny Comparative genomics Genomics Sequence Analysis DNA General Medicine biology.organism_classification Jumbo phage Viral replication GenBank GC-content |
| Zdroj: | DNA Research, 28(4) DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes |
| ISSN: | 1756-1663 1340-2838 |
| DOI: | 10.1093/dnares/dsab013 |
| Popis: | Bacteriophages are an invaluable source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses as biotechnological and medical tools, and help unravel the diversity of biological mechanisms employed by phages to take over the host during viral infection. Aiming to expand the available collection of phage genomes, we have isolated, sequenced, and assembled the genome sequences of four phages that infect the clinical pathogen Klebsiella pneumoniae: vB_KpnP_FBKp16, vB_KpnP_FBKp27, vB_KpnM_FBKp34, and Jumbo phage vB_KpnM_FBKp24. The four phages show very low (0–13%) identity to genomic phage sequences deposited in the GenBank database. Three of the four phages encode tRNAs and have a GC content very dissimilar to that of the host. Importantly, the genome sequences of the phages reveal potentially novel DNA packaging mechanisms as well as distinct clades of tubulin spindle and nucleus shell proteins that some phages use to compartmentalize viral replication. Overall, this study contributes to uncovering previously unknown virus diversity, and provides novel candidates for phage therapy applications against antibiotic-resistant K. pneumoniae infections. |
| Databáze: | OpenAIRE |
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