Expression of the class II tumor suppressor gene RIG1 is directly regulated by p53 tumor suppressor in cancer cell lines
| Autor: | May-Whey Hung, Wen-Chieh Ni, Tsu-Chung Chang, Huai-En Lin, Shun-Yuan Jiang, Tzu-Hui Hsu, Chin-Chen Chu |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
p53
Transcriptional Activation Tumor suppressor gene Transcription Genetic Receptors Retinoic Acid Biophysics Tazarotene-induced gene 3 Biology Retinoid-inducible gene 1 Response Elements Biochemistry Retinoblastoma-like protein 1 Transactivation Class II tumor suppressor Structural Biology Cell Line Tumor Genetics SOCS5 E2F1 Humans SOCS6 Genes Tumor Suppressor Molecular Biology Regulation of gene expression Retinoic acid receptor responder 3 Base Sequence Cell Biology Molecular biology GPS2 Gene Expression Regulation Neoplastic Tumor Suppressor Protein p53 |
| Zdroj: | FEBS letters. 586(9) |
| ISSN: | 1873-3468 |
| Popis: | Recent studies indicated that the RIG1 (RARRES3/TIG3) plays an important role in cell proliferation, differentiation, and apoptosis. However, the regulatory mechanism of RIG1 gene expression has not been clearly elucidated. In this study, we identified a functional p53 response element (p53RE) in the RIG1 gene promoter. Transfection studies revealed that the RIG1 promoter activity was greatly enhanced by wild type but not mutated p53 protein. Sequence specific mutation of the p53RE abolished p53-mediated transactivation. Specific binding of p53 protein to the rig-p53RE was demonstrated using electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay. Further studies confirmed that the expression of RIG1 mRNA and protein is enhanced through increased p53 protein in HepG2 or in H24-H1299 cells. In conclusion, our results indicated that RIG1 gene is a downstream target of p53 in cancer cell lines. |
| Databáze: | OpenAIRE |
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