Identification of potential biomarkers for systemic lupus erythematosus diagnosis using two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry

Autor:	Ivana Helena Rocha Oliveira, Hélida Monteiro de Andrade, Luis Carlos Fialho Júnior, Simone da Fonseca Pires, Donat Alexander Chapeourouge, Bruna Soares de Souza Lima, Walter Batista Cicarini, Vicente de Paulo Coelho Peixoto de Toledo, Tamara Aparecida Reis Ferreira, Tânia Mara Pinto Dabés Guimarães, Maria das Graças Carvalho, Jonas Perales, Paulo Madureira de Pádua
Rok vydání:	2017
Předmět:	0301 basic medicine Adult Male Proteomics medicine.medical_specialty Immunology Mass Spectrometry Autoimmune Diseases Two-Dimensional Difference Gel Electrophoresis 03 medical and health sciences Young Adult Immune system immune system diseases Internal medicine medicine Immunology and Allergy Humans Lupus Erythematosus Systemic skin and connective tissue diseases Autoimmune disease biology Autoantibody Middle Aged medicine.disease Rheumatology Complement system Transthyretin 030104 developmental biology Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Female Biomarkers Anti-SSA/Ro autoantibodies
Zdroj:	Autoimmunity. 50(4)
ISSN:	1607-842X
Popis:	Systemic lupus erythematosus (SLE) is an autoimmune disease of the connective tissue with a large spectrum of clinical manifestations. Immune deregulation leads to autoantibody and immune complexes overproduction, complement activation, and persistent tissue inflammation. Considering that the current diagnosis depends on the interpretation of the complex criteria established by the American College of Rheumatology and that the disease course is characterized by unpredictable activations and remissions, each patient develops different manifestations, and therefore, the discovery of specific biomarkers is urgently required. Therefore, this study aimed to identify putative biomarkers for active and inactive SLE potentially capable in distinguishing laboratorial SLE from other autoimmune diseases. The 2D-DIGE proteomics technique was used to evaluate the differential abundance of proteins between patients with active SLE, inactive SLE, patients with other autoimmune disease, and healthy individuals. Six proteins showed increased abundance in active SLE (A) and inactive SLE (I) compared to the C and O groups, but not between groups A and I. There were two transthyretin (TTR) fragments or proteins with a structure similar to TTR (accession numbers: PDB: 1GKO_A and 2PAB_A), retinol-binding protein 4 (RBP4) isoform X1 (no information in databases such as UNIPROT), and antibody fragments. Two proteins, APO-AIV and SP-40,40, were upregulated in group A than in O and C and in group I versus C, but not in group I versus O. Therefore, we suggest these proteins to be considered as candidates for the diagnosis of SLE.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23cfe72dc55001ff95bbe41c5a076f49 https://pubmed.ncbi.nlm.nih.gov/28675715 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.