3D printing of Microgel-loaded Modular LEGO-like Cages as Instructive Scaffolds for Tissue Engineering
| Autor: | James M. Jones, Anthony Tahayeri, Christina Hipfinger, Avathamsa Athirasala, Luiz E. Bertassoni, Amin Mansoorifar, Diana Araujo Cunha, Sivaporn Horsophonphong, Ramesh Subbiah, Cristiane Miranda França, Robert E. Guldberg, Albena Zahariev, Greeshma Thrivikraman, Hua Xie, Paulo G. Coelho, Lukasz Witek |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
business.industry Computer science 3D printing Biomaterial Nanotechnology 02 engineering and technology Modular design 021001 nanoscience & nanotechnology Regenerative medicine 03 medical and health sciences Tissue engineering Tissue formation 0210 nano-technology business Lithography 030304 developmental biology |
| DOI: | 10.1101/2020.03.02.974204 |
| Popis: | Biomaterial scaffolds have served as the foundation of tissue engineering and regenerative medicine. However, scaffold systems are often difficult to scale in size or shape in order to fit defect-specific dimensions, and thus provide only limited spatiotemporal control of therapeutic delivery and host tissue responses. Here, a lithography-based three-dimensional (3D) printing strategy is used to fabricate a novel miniaturized modular LEGO-like cage scaffold system, which can be assembled and scaled manually with ease. Scalability is based on an intuitive concept of stacking modules, like conventional LEGO blocks, allowing for literally thousands of potential geometric configurations, and without the need for specialized equipment. Moreover, the modular hollow-cage design allows each unit to be loaded with biologic cargo of different compositions, thus enabling controllable and easy patterning of therapeutics within the material in 3D. In summary, the concept of miniaturized cage designs with such straight-forward assembly and scalability, as well as controllable loading properties, is a flexible platform that can be extended to a wide range of materials for improved biological performance.TOC3D printed LEGO-like hollow microcages can be easily assembled, adjoined, and stacked-up to suit the complexity of defect tissues; aid spatial loading of cells and biomolecules; instruct cells migration three-dimensionally; and facilitate cell invasion and neovascularization in-vivo, thus accelerating the process of tissue healing and new tissue formation. |
| Databáze: | OpenAIRE |
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