Vascular Patterning as Integrative Readout of Complex Molecular and Physiological Signaling by VESsel GENeration Analysis
| Autor: | Moldovan Nicanor I, Alexander Pinhas, Susana B. Zanello, Corey A. Theriot, Mark Lagatuz, Miguel Martinho, Giovanni Taibbi, Toco Yuen Ping Chui, Patricia Parsons-Wingerter, Daniel J. Lindner, Ruchi J. Vyas, Maria B. Grant, Shiyin Lim, Nicole M. Jacobs, Krishnan Radhakrishnan, Mariana Dupont, Marina Predovic, Hans-Christian Reinecker, Nikunj C. Oza, Richard B Rosen, Mary B. Vickerman, David L. Kao, Hamed Valizadegan, Gianmarco Vizzeri |
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| Rok vydání: | 2021 |
| Předmět: |
Models
Anatomic 0301 basic medicine Physiology Angiogenesis Basic fibroblast growth factor Neovascularization Physiologic Inflammation Vascular Remodeling 030204 cardiovascular system & hematology Biology Article law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Retinal Vein Occlusion medicine Animals Humans Computer Simulation Angiogenic Proteins 3D bioprinting Retina Diabetic Retinopathy Neovascularization Pathologic Weightlessness Bioprinting Models Cardiovascular Retinal Vessels Arteries Biomarker (cell) Vascular endothelial growth factor Fractals 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation chemistry Printing Three-Dimensional Astronauts Keratinocyte growth factor medicine.symptom Cardiology and Cardiovascular Medicine Neuroscience Software Signal Transduction |
| Zdroj: | J Vasc Res |
| ISSN: | 1423-0135 1018-1172 |
| DOI: | 10.1159/000514211 |
| Popis: | The molecular signaling cascades that regulate angiogenesis and microvascular remodeling are fundamental to normal development, healthy physiology, and pathologies such as inflammation and cancer. Yet quantifying such complex, fractally branching vascular patterns remains difficult. We review application of NASA’s globally available, freely downloadable VESsel GENeration (VESGEN) Analysis software to numerous examples of 2D vascular trees, networks, and tree-network composites. Upon input of a binary vascular image, automated output includes informative vascular maps and quantification of parameters such as tortuosity, fractal dimension, vessel diameter, area, length, number, and branch point. Previous research has demonstrated that cytokines and therapeutics such as vascular endothelial growth factor, basic fibroblast growth factor (fibroblast growth factor-2), transforming growth factor-beta-1, and steroid triamcinolone acetonide specify unique “fingerprint” or “biomarker” vascular patterns that integrate dominant signaling with physiological response. In vivo experimental examples described here include vascular response to keratinocyte growth factor, a novel vessel tortuosity factor; angiogenic inhibition in humanized tumor xenografts by the anti-angiogenesis drug leronlimab; intestinal vascular inflammation with probiotic protection by Saccharomyces boulardii, and a workflow programming of vascular architecture for 3D bioprinting of regenerative tissues from 2D images. Microvascular remodeling in the human retina is described for astronaut risks in microgravity, vessel tortuosity in diabetic retinopathy, and venous occlusive disease. |
| Databáze: | OpenAIRE |
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