High prevalence of DS-1-like rotavirus infection in Thai adults between 2016 and 2019

Autor: Watchaporn Chuchaona, Siripat Pasittungkul, Fajar Budi Lestari, Yong Poovorawan, Sompong Vongpunsawad, Jira Chansaenroj, Chintana Chirathaworn, Sirapa Klinfueng, Nasamon Wanlapakorn
Jazyk: angličtina
Rok vydání: 2020
Předmět:
RNA viruses
Rotavirus
Male
0301 basic medicine
Viral Diseases
viruses
Pathology and Laboratory Medicine
medicine.disease_cause
Geographical Locations
Feces
fluids and secretions
Reoviruses
DS-1
Genotype
Medicine and Health Sciences
Prevalence
Antigens
Viral
Phylogeny
Data Management
Aged
80 and over
Vaccines
Multidisciplinary
High prevalence
Database and informatics methods
Incidence (epidemiology)
Sequence analysis
virus diseases
Phylogenetic Analysis
Middle Aged
Thailand
Gastroenteritis
Phylogenetics
Rotavirus infection
Infectious Diseases
Medical Microbiology
Viral Pathogens
Viruses
RNA
Viral
Medicine
Female
Pathogens
Research Article
Adult
Computer and Information Sciences
Asia
Infectious Disease Control
Adolescent
Bioinformatics
Science
030106 microbiology
Nucleotide Sequencing
Gastroenterology and Hepatology
Research and Analysis Methods
Microbiology
Rotavirus Infections
Young Adult
03 medical and health sciences
medicine
Humans
Evolutionary Systematics
Molecular Biology Techniques
Sequencing Techniques
Molecular Biology
Microbial Pathogens
Genotyping
Rotavirus Infection
DNA sequence analysis
Taxonomy
Aged
Evolutionary Biology
business.industry
Organisms
Biology and Life Sciences
Outbreak
Virology
030104 developmental biology
People and Places
Capsid Proteins
business
Zdroj: PLoS ONE, Vol 15, Iss 6, p e0235280 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Rotavirus infection is the most common cause of viral diarrhea in infants and young children but uncommon and usually asymptomatic in adults. In the winter of 2017-2018, a large-scale outbreak of rotavirus in both children and adults was reported in Thailand. The current study focused on the prevalence, genotyping, and molecular characterization of rotavirus infections in Thai adults from July 2016 to December 2019. In 2,598 stool samples collected from adult residents of Bangkok (aged #x2265; 15 years) with acute gastroenteritis, rotavirus was detected via real-time RT-PCR analysis of the VP6 gene. G, P and I genotypes were determined by direct sequencing of VP7, VP4, and VP6 genes, respectively. Our results showed 8.7% (226/2,598) of stool samples were positive for rotavirus. The incidence of rotavirus was high during the winter season of 2017-2018 (17.7%) compared to another studied periods (4.5% between July 2016- October 2017 and 2.8% between March 2018- December 2019). Nucleotide sequencing of VP7 and VP4 revealed G3P[8] as the predominant strain (33.2%,75/226), followed by G9P[8] (17.3%,39/226), and G2P[4] (15.0%,34/226). Uncommon G and P combinations were additionally detected at low frequencies. VP6 sequencing was conducted to discriminate I genotype between the Wa and DS-1 genogroup. The unusual DS-1-like G3P[8] strain was most prevalent amomg rotavirus strains detected in this study (29.6%, 67/226), and the corresponding VP7 sequences showed high nucleotide identity with unusual DS-1-like globally circulating strains. Our study demonstrates that rotavirus outbreaks in adults are attributable not only to high prevalence of RV infection but also the unusual DS-like genogroup. The collective findings reinforce the importance of investigating rotavirus diagnosis in adults suffering from acute gastroenteritis and taking appropriate preventive measures.
Databáze: OpenAIRE
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