Low concentration of ethanol favors progenitor cell differentiation and neovascularization in high-fat diet-fed mice model

Autor: Raffaella Soleti, Luisa Vergori, Emilie Lauret, Maria Carmen Martinez, Ramaroson Andriantsitohaina
Přispěvatelé: Stress Oxydant et Pathologies Métaboliques (SOPAM), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Andriantsitohaina, Ramaroson
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Normal diet
Endothelium
Cell Survival
Angiogenesis
Neovascularization
Physiologic
Bone Marrow Cells
030204 cardiovascular system & hematology
Biology
Diet
High-Fat
Biochemistry
Neovascularization
Mice
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
Internal medicine
medicine
Animals
Progenitor cell
ComputingMilieux_MISCELLANEOUS
Progenitor
2. Zero hunger
Dose-Response Relationship
Drug
Ethanol
Stem Cells
Regeneration (biology)
Body Weight
Cell Differentiation
Fasting
Cell Biology
[SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Immunology
Cytokines
Bone marrow
medicine.symptom
Zdroj: International Journal of Biochemistry and Cell Biology
International Journal of Biochemistry and Cell Biology, Elsevier, 2016, ⟨10.1016/j.biocel.2016.07.004.[Epubaheadofprint]⟩
International Journal of Biochemistry and Cell Biology, Elsevier, 2016, ⟨10.1016/j.biocel.2016.07.004. [Epub ahead of print]⟩
ISSN: 1357-2725
Popis: Endothelial progenitor cells (EPCs) and monocytic cells from bone marrow (BM) can be recruited to the injured endothelium and contribute to its regeneration. During metabolic diseases such as obesity and diabetes, progenitor cell function is impaired. Several studies have shown that moderate alcohol consumption prevents the development and progression of atherosclerosis in a variety of animal/mouse models and increases mobilization of progenitor cells. Along with these studies, we identify ethanol at low concentration as therapeutic tool to in vitro expand progenitor cells in order to obtain an adequate number of cells for their use in the treatment of cardiovascular diseases. We evaluated the effects of ethanol on the phenotype of BM-derived cells from mice fed with high-fat diet (HFD). HFD did not induce changes in weight of mice but induced metabolic alterations. HFD feeding increased the differentiation of monocytic progenitors but not EPCs. Whereas ethanol at 0.6% is able to increase monocytic progenitor differentiation, 1% ethanol diminished it. Furthermore, ethanol at 0.6% increased the ability of progenitor cells to promote in vivo angiogenesis as well as secretome of BM-derived cells from mice fed with HFD, but not in mice fed normal diet. In conclusion, ethanol at low concentration is able to increase angiogenic abilities of progenitor cells from animals with early metabolic alterations.
Databáze: OpenAIRE
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