Role of NF-κβ and Oxidative Pathways in Atherosclerosis: Cross-Talk Between Dyslipidemia and Candesartan

Autor:	Fadhil G. Al-Amran, Bassim I Mohammad, Nasser Ghaly Yousif, Najah R. Hadi, Mohammed Saeed Abdulzahra, Murooge L. Majeed, Maitham G. Yousif
Rok vydání:	2013
Předmět:	medicine.medical_specialty Chemokine Normal diet Tetrazoles Vascular Cell Adhesion Molecule-1 medicine.disease_cause Monocytes chemistry.chemical_compound Cell Movement Internal medicine medicine Animals Pharmacology (medical) Dyslipidemias Pharmacology biology business.industry Biphenyl Compounds NF-kappa B General Medicine Glutathione Atherosclerosis medicine.disease Malondialdehyde Lipids Candesartan Endocrinology Atheroma chemistry biology.protein Cytokines Benzimidazoles Rabbits Chemokines Cardiology and Cardiovascular Medicine business Angiotensin II Type 1 Receptor Blockers Oxidation-Reduction Dyslipidemia Oxidative stress medicine.drug
Zdroj:	Cardiovascular Therapeutics. 31:381-387
ISSN:	1755-5914
Popis:	Summary Purpose The objective of this study is to assess the effect of the candesartan on the progression of atherosclerosis through the downregulation of NF-κβ and interference with oxidative pathway. Methods Twenty-four rabbits were assigned to three groups: control group fed normal diet; induced untreated group fed 1% cholesterol diet; and treated candesartan group also fed 1% cholesterol diet. Plasma lipid profiles were measured, and ELISA for plasma cytokines and chemokine was performed. Analyses of NF-κβ and VCAM-1 were performed using Western blotting with RT-PCR for NF-κB activity at mRNA. Doppler ultrasound was used to evaluate aortic intima-media thickness, and atheroma was detected by H&E staining. Immunofluorescent staining was performed to confirm accumulation of monocytes and PMNs. Results Candesartan markedly reduced the levels of the plasma lipid profile including total cholesterol [TC], triglycerides [TG], and LDL-C, while significantly elevating levels in the plasma HDL-C, in addition to reducing cytokine (TNF-α, IL-6, IL-1β) and chemokine levels (MCP-1). Also, it decreased the aortic malondialdehyde (MDA) concentration and elevated the aortic glutathione (GSH) level compared with untreated animals (P
Databáze:	OpenAIRE
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