Effects of Photobiomodulation Therapy (LED 630 nm) on Muscle and Nerve Histomorphometry after Axonotmesis
| Autor: | Giovanna Moura Lamas Della Santa, Murilo Xavier Oliveira, Ana Paula Santos, Marcílio Coelho Ferreira, Thaís Peixoto Gaiad Machado |
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| Rok vydání: | 2020 |
| Předmět: |
Biochemistry
030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Axonotmesis Medicine Animals Trauma Nervous System Physical and Theoretical Chemistry Axon Low-Level Light Therapy Muscle Skeletal business.industry Regeneration (biology) Skeletal muscle General Medicine Anatomy medicine.disease Median nerve Peripheral Staining Nerve Regeneration Rats medicine.anatomical_structure Myelin sheath business 030217 neurology & neurosurgery |
| Zdroj: | Photochemistry and photobiologyREFERENCES. 97(5) |
| ISSN: | 1751-1097 |
| Popis: | Peripheral injuries constitute a substantial clinical problem with unsatisfactory treatment. The study's objective was to analyze the effects of photobiomodulation therapy (PBMT) on median nerve regeneration and muscle recovery after axonotmesis. Twenty-four rats were randomized into three groups: control (CG), injury (IG), and LED therapy (LEDG). A 630 ± 20 nm (300-mW) LED was placed in contact with the skin. One point over the injury site was irradiated for 30 s, delivering 9 J (9 J cm-2 ). PBMT irradiation was performed once daily for 5 days followed by two-day interval and then more five consecutive days of treatment. Proximal and distal segments of the nerve and flexors muscles were removed for histomorphometric analysis using H&E staining for muscles and osmium tetroxide for nerves. The myelinated fiber and axon diameter and the myelin sheath thickness were greater in the proximal and distal nerve segments in the LEDG compared to the IG (P ≤ 0.05). The number of myelinated fibers was greater in the distal segment of the LEDG (P ≤ 0.05). The area, circumference, and diameter of the muscle fibers were larger in the LEDG than in the IG (P ≤ 0.05). The PBMT protocol used favored axonal regeneration and muscle recovery. |
| Databáze: | OpenAIRE |
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