Identification of serum miR-139-3p as a non-invasive biomarker for colorectal cancer
| Autor: | Ariel K.M. Chow, Thomas Yau, Wai Lun Law, Roberta Pang, Oswens Siu-Hung Lo, Johnny Hon Wai Man, Timothy Ming-Hun Wan, Guanghua Chen, Dominic Chi-Chung Foo, Deepak Narayanan Iyer, Wai-Keung Leung, Lui Ng, Jensen T. C. Poon |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Adult Male medicine.medical_specialty Pathology Colorectal cancer 03 medical and health sciences 0302 clinical medicine Internal medicine Tumor stage medicine Biomarkers Tumor Humans Liquid biopsy Neoplasm Metastasis Early Detection of Cancer Aged Neoplasm Staging miRNA miR-139-3p business.industry Gene Expression Profiling Non invasive biomarkers Case-control study Liquid Biopsy miR-622 Middle Aged medicine.disease Control subjects Tumor Burden CRC MicroRNAs 030104 developmental biology Real-time polymerase chain reaction ROC Curve 030220 oncology & carcinogenesis Case-Control Studies Biomarker (medicine) biomarker Female business Colorectal Neoplasms Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Lui Ng 1, * , Timothy Ming-Hun Wan 1, * , Johnny Hon-Wai Man 1 , Ariel Ka-Man Chow 1 , Deepak Iyer 1 , Guanghua Chen 1 , Thomas Chung-Cheung Yau 2 , Oswens Siu-Hung Lo 1 , Dominic Chi-Chung Foo 1 , Jensen Tung-Chung Poon 1 , Wai-Keung Leung 3 , Roberta Wen-Chi Pang 1, 2 , Wai-Lun Law 1 1 Department of Surgery, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China 2 Centre for Cancer Research, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China 3 Department of Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China * These authors have contributed equally to this work Correspondence to: Wai-Lun Law, email: lawwl@hku.hk Roberta Wen-Chi Pang, email: luing@hku.hk Keywords: miR-139-3p, miR-622, CRC, miRNA, biomarker Abbreviations: CRC, colorectal cancer Received: May 16, 2016 Accepted: January 16, 2017 Published: March 14, 2017 ABSTRACT Aberrant levels of circulating microRNAs are potential biomarkers for the early detection of colorectal cancer. The aim of this study was to study miR-139-3p and miR-622 in serum as a non-invasive biomarker for colorectal cancer diagnosis. We applied quantitative polymerase chain reaction to determine the levels of miR-139-3p and miR-622 in 42 pairs of tumor and adjacent non-tumor tissues, and in serum samples of 117 patients and 90 control subjects. Our results showed that miR-139-3p was silenced whereas miR-622 was overexpressed in colorectal cancer. Similarly, serum miR-139-3p level was significantly lower in colorectal cancer patients than in control subjects whereas miR-622 was more frequently detectable in patients. ROC analysis showed that AUC of miR-139-3p was 0.9935, with a sensitivity of 96.6% and specificity of 97.8%. Serum miR-139-3p level showed high sensitivity and specificity for both early and late stage CRCs and proximal and distal CRCs. Detectable serum miR-622 showed a sensitivity of 87.5% and specificity of 63.5% for discriminating CRC patients, but the sensitivity dropped for late stage patients (72.7%). We also included analyses of the blood CEA level for comparing the diagnostic performance of these blood-based biomarkers. The median level in CRC patients (3.6 ng/ml) was significantly higher than that in control (1.8 ng/ml). The AUC value of CEA in diagnosing CRC patients was 0.7515. CEA showed a positive correlation with tumor stage and age of patients and its level was higher in male. Collectively, serum miR-139-3p has strong potential as a promising non-invasive biomarker in colorectal cancer detection. |
| Databáze: | OpenAIRE |
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