Rotaviruses Associate with Distinct Types of Extracellular Vesicles
| Autor: | Ivan R. Quevedo, Pavel Isa, Susana López, Carlos F. Arias, Arianna Pérez-Delgado |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Rotavirus viruses 030106 microbiology Cell lcsh:QR1-502 exosomes medicine.disease_cause Virus lcsh:Microbiology Article Rotavirus Infections Cell membrane 03 medical and health sciences Extracellular Vesicles fluids and secretions Microscopy Electron Transmission Virology medicine Humans Secretion Virus Release Chemistry Vesicle Virion virus diseases Microvesicles 030104 developmental biology Infectious Diseases medicine.anatomical_structure Cell culture Caco-2 Cells microvesicles |
| Zdroj: | Viruses Viruses, Vol 12, Iss 763, p 763 (2020) Volume 12 Issue 7 |
| ISSN: | 1999-4915 |
| Popis: | Rotaviruses are the leading cause of viral gastroenteritis among children under five years of age. Rotavirus cell entry has been extensively studied however, rotavirus cell release is still poorly understood. Specifically, the mechanism by which rotaviruses leave the cell before cell lysis is not known. Previous works have found rotavirus proteins and viral particles associated with extracellular vesicles secreted by cells. These vesicles have been shown to contain markers of exosomes however, in a recent work they presented characteristics more typical of microparticles, and they were associated with an increase in the infectivity of the virus. In this work, we purified different types of vesicles from rotavirus-infected cells. We analyzed the association of virus with these vesicles and their possible role in promotion of rotavirus infection. We confirmed a non-lytic rotavirus release from the two cell lines tested, and observed a notable stimulation of vesicle secretion following rotavirus infection. A fraction of the secreted viral particles present in the cell supernatant was protected from protease treatment, possibly through its association with membranous vesicles the more pronounced association of the virus was with fractions corresponding to cell membrane generated microvesicles. Using electron microscopy, we found different size vesicles with particles resembling rotaviruses associated from both- the outside and the inside. The viral particles inside the vesicles were refractory to neutralization with a potent rotavirus neutralizing monoclonal antibody, and were able to infect cells even without trypsin activation. The association of rotavirus particles with extracellular vesicles suggests these might have a role in virus spread. |
| Databáze: | OpenAIRE |
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