Postnatal high-protein diet improves learning and memory in premature rats via activation of mTOR signaling
| Autor: | Jia-yi Liao, Fan Wu, Weiwen Sun, Qiliang Cui, Fei-Tong Zhang, Tao Zhang, Zhiwen Su, Xiao-Hua Tian, Hui Zhang |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Normal diet Spatial Learning Morris water navigation task Hippocampus P70-S6 Kinase 1 High-protein diet Biology medicine.disease_cause Rats Sprague-Dawley Internal medicine medicine Animals Maze Learning Molecular Biology PI3K/AKT/mTOR pathway Spatial Memory General Neuroscience TOR Serine-Threonine Kinases Rats Endocrinology Animals Newborn Ribosomal protein s6 Phosphorylation Neurology (clinical) Dietary Proteins Developmental Biology Signal Transduction |
| Zdroj: | Brain research. 1611 |
| ISSN: | 1872-6240 |
| Popis: | Purpose The present study investigated whether a high-protein diet affects spatial learning and memory in premature rats via modulation of mammalian target of rapamycin (mTOR) signaling. Methods Pre- and full-term Sprague-Dawley pups were fed a normal (18% protein) or high-protein (30% protein) diet (HPD) for 6 or 8 weeks after weaning. Spatial learning and memory were tested in the Morris water maze at week 6 and 8. The activation of mTOR signaling pathway components was evaluated by western blotting. Results Spatial memory performance of premature rats consuming a normal and HPD was lower than that of full-term rats on the same diet at 6 weeks, and was associated with lower levels of ribosomal protein S6 kinase p70 subtype (p70S6K) and initiation factor 4E-binding protein 1 (4EBP1) phosphorylation in the hippocampus. Spatial memory was improved in 8-week-old premature rats on an HPD as compared to those on a normal diet. Premature rats on an HPD had p70S6K and 4EBP1 phosphorylation levels in the hippocampus that were comparable to those of full-term rats on an HPD. Conclusion Long-term consumption of a protein-rich diet can restore the impairment in learning and memory in pre-term rats via upregulation of mTOR/p70S6K signaling. |
| Databáze: | OpenAIRE |
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