Characterization of Arabinosyl Transfer Reactions in the Biosynthesis of Mycobacterial Cell Envelope (Lipo)Polysaccharides
| Autor: | Mary Jackson, Shiva K. Angala |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Glycan 030106 microbiology Mycobacterium smegmatis Polysaccharide Galactans Mass Spectrometry Article Substrate Specificity 03 medical and health sciences chemistry.chemical_compound Biosynthesis Bacterial Proteins Arabinogalactan Cell Wall Glycosyl Pentosyltransferases Enzyme Assays chemistry.chemical_classification Lipoarabinomannan biology Chemistry Arabinose Biosynthetic Pathways carbohydrates (lipids) 030104 developmental biology Enzyme Biochemistry biology.protein Cell envelope Chromatography Liquid |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493991532 |
| ISSN: | 1940-6029 |
| Popis: | D-Arabinofuranose is a major glycosyl constituent of mycobacteria found in two essential cell envelope heteropolysaccharides, arabinogalactan and lipoarabinomannan. Seven different arabinosyltransferases at least are required to synthesize the arabinan domain of these two major glycans. Because of their interest from the perspective of drug development, these enzymes have been the object of intense investigations. In this chapter, we describe the protocols used to perform nonradioactive arabinosyltransferase assays with synthetic acceptor and donor substrates and characterize the enzymatic products of the reactions by mass spectrometry. |
| Databáze: | OpenAIRE |
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