A Dual-Sensing Receptor Confers Robust Cellular Homeostasis
| Autor: | Kirsten Jung, Hannah Schramke, Ralf Heermann, Filipe Tostevin, Ulrich Gerland |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030106 microbiology Phosphatase Cellular homeostasis Biology Models Biological General Biochemistry Genetics and Molecular Biology Protein Structure Secondary 03 medical and health sciences Stress Physiological Extracellular Escherichia coli Homeostasis Amino Acid Sequence Receptor lcsh:QH301-705.5 Kinase Escherichia coli Proteins Temperature Robustness (evolution) Phosphoric Monoester Hydrolases ddc Cell biology 030104 developmental biology Biochemistry lcsh:Biology (General) Periplasm Potassium Mutant Proteins Protein Kinases Intracellular |
| Zdroj: | Cell Reports, Vol 16, Iss 1, Pp 213-221 (2016) |
| ISSN: | 2211-1247 |
| Popis: | SummaryCells have evolved diverse mechanisms that maintain intracellular homeostasis in fluctuating environments. In bacteria, control is often exerted by bifunctional receptors acting as both kinase and phosphatase to regulate gene expression, a design known to provide robustness against noise. Yet how such antagonistic enzymatic activities are balanced as a function of environmental change remains poorly understood. We find that the bifunctional receptor that regulates K+ uptake in Escherichia coli is a dual sensor, which modulates its autokinase and phosphatase activities in response to both extracellular and intracellular K+ concentration. Using mathematical modeling, we show that dual sensing is a superior strategy for ensuring homeostasis when both the supply of and demand for a limiting resource fluctuate. By engineering standards, this molecular control system displays a strikingly high degree of functional integration, providing a reference for the vast numbers of receptors for which the sensing strategy remains elusive. |
| Databáze: | OpenAIRE |
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