Separation of transition metals on a poly-iminodiacetic acid grafted polymeric resin column with post-column reaction detection utilising a paired emitter–detector diode system
| Autor: | Pavel N. Nesterenko, Martina O’Toole, Leon Barron, Dermot Diamond, Brett Paull |
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| Rok vydání: | 2008 |
| Předmět: |
Polymers
Iminodiacetic acid Analytical chemistry chemistry.chemical_element Zinc Resorcinol Picolinic acid Biochemistry Chromatography Affinity Analytical Chemistry Metal chemistry.chemical_compound Transition metal Transition Elements Detection limit Chromatography Magnesium Imino Acids Osmolar Concentration Organic Chemistry Temperature Resorcinols General Medicine chemistry visual_art visual_art.visual_art_medium |
| Zdroj: | Journal of Chromatography A. 1213:31-36 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2008.08.049 |
| Popis: | The selectivity, retention and separation of transition metals on a short (2 mm x 50 mm) column packed with a poly-iminodiacetic acid functionalised polymer 10 microm resin (Dionex ProPac IMAC-10) are presented. This stationary phase, typically used for the separation of proteins, is composed of long chain poly-iminodiacetic acid groups grafted to a hydrophilic layer surrounding a 10 microm polymeric bead. Through the use of a combination of a multi-step pH and picolinic acid gradient, the separation of magnesium, iron, cobalt, cadmium, zinc, lead and copper was possible, followed by post-column reaction with 4-(2-pyridylazo) resorcinol (PAR) and absorbance detection at 510 nm using a novel and inexpensive optical detector, comprised of two light emitting diodes with one acting as a light source and the other as a detector. Column efficiency for selective transition metals was in excess of N=10,000, with the baseline separation of seven metal cations in3 min possible under optimised conditions. Detection limits of between 5 and 81 microg/L were possible based upon a 50 microL injection volume. |
| Databáze: | OpenAIRE |
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